烟草甲线粒体基因组序列测定与分析  

Sequencing and analysis of mitochondrial genome of Lasioderma serricorne

作　　者：夏丽媛 孙为伟 崔淼 伍祎 XIA Liyuan;SUN Weiwei;CUI Miao;WU Yi(Academy of National Food and Strategic Reserves Administration,Beijing 100037,China;Collaborative Innovation Center of Henan Food Crops,Zhengzhou 450002,China;Jiangsu Collaborative Innovation Center for Modern Grain Circulation and Safety,Nanjing 210023,China)

机构地区：[1]国家粮食和物资储备局科学研究院,北京市西城区100037 [2]河南粮食作物协同创新中心,郑州市金水区450002 [3]江苏省现代粮食流通与安全协同创新中心,南京市玄武区210023

出　　处：《烟草科技》2020年第12期1-8,共8页Tobacco Science & Technology

基　　金：全国粮食行业青年拔尖人才服务行业需求自主选题项目“储粮虫螨形态和分子信息体系研究”(RC1802);国家粮食和物资储备局科学研究院自主选题“主要储粮虫螨线粒体基因组研究”(ZX1917-1);国家重点研发计划“微小储粮害虫绿色治理关键技术和设备研究”(2016YFD0401004-2)。

摘　　要：为进一步获取烟草甲线粒体基因组信息,以期为烟草甲分类鉴定、系统发育和种群遗传等方面的深入研究奠定基础,采用二代测序技术测定了烟草甲线粒体基因组序列。结果表明:烟草甲线粒体基因组全长15009 bp,包含13个蛋白质编码基因、2个核糖体RNA基因、22个转运RNA基因和1个控制区,基因组排列的顺序和组成与鞘翅目储粮害虫线粒体基因组相似。烟草甲线粒体基因组的A+T含量为78.7%,13个蛋白质编码基因除cox2基因的起始密码子是TTG外,其余蛋白质编码基因的起始密码子均为通用密码子ATN;典型的TAA或TAG为9个基因的终止密码子,而cox3、nad5、nad4以不完全的T作为终止密码。烟草甲的22个tRNA均能折叠成三叶草结构,但tRNAAsp、tRNALeu和tRNAHis的二级结构中缺少TψC环,tRNAser(AGN)的DHU(Dihydorouridine,二氢尿嘧啶)臂缺失,因而不能形成典型的三叶草式二级结构。To further study the genetic information and to promote the research on identification,phylogeny and population genetics of Lasioderma serricorne,the next-generation sequencing was used to acquire the complete mitochondrial genome of L.serricorne.The results showed that the mitochondrial genome of L.serricorne was 15009 bp in length,which contained 13 protein-coding genes,2 ribosomal RNA genes,22 translocation RNA genes and a control region.The structure and organization of the genome were similar with those of the stored-product pests from Coleoptera.The A+T content of the whole mitochondrial genome was 78.7%.Except for cox2 whose start codon was TTG,the start codons of protein-coding genes were typical ATN codon.The stop codons of cox3,nad5,nad4 were incomplete T,and the other genes used the typical TAA or TAG as the stop codons.The 22 tRNA of L.serricorne could fold into a clover structure,however tRNAAsp,tRNALeu,tRNAHis and tRNAse(r AGN)folded into atypical cloverleaf secondary structures with the absence of either TψC-arm or D-arm.

关 键 词：窃蠹科 烟草甲 线粒体基因组 二代测序 

分 类 号：Q963[生物学—昆虫学]