Systematic Identification of the Light-quality Responding Anthocyanin Synthesis-related Transcripts in Petunia Petals  

作　　者：Zhenzhu Fu Hongquan Shang Hui Jiang Jie Gao Xiaoyu Dong Huijuan Wang Yanmin Li Limin Wang Jing Zhang Qingyan Shu Yacong Chao Menglan Xu Rui Wang Liangsheng Wang Hechen Zhang 

机构地区：[1]Horticultural Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China [2]Key Laboratory of Plant Resources/Beijing Botanical Garden,Institute of Botany,The Chinese Academy of Sciences,Beijing 100093,China [3]College of Forestry,Henan Agricultural University,Zhengzhou 450002,China

出　　处：《Horticultural Plant Journal》2020年第6期428-438,共11页园艺学报（英文版）

基　　金：This research was supported by the National Natural Science Foundation of China(Grant No.U1504320);Financial Project of Henan Province(Grant No.2019ZC23);We thank Liwen Bianji,Edanz Group China(www.liwenbianji.cn/ac),for editing the English text of a draft of this manuscript.

摘　　要：Previous studies have shown that high light intensity can induce anthocyanin synthesis(AS)in petunia plants.To identifywhich kind of light quality plays a role in inducing such metabolic process,and what transcripts participate in controlling it,we carried out whole-transcriptome sequencing and analysis of petunia petals treated with different light-quality conditions.Among the red and white light treatments,a total of 2205 differentially expressed genes and 15,22,and 20 differentially expressed circRNAs,miRNAs,and lncRNAs,were identified respectively.The AS-related genes,including the structural genes CHSj,F3H,F35H,DFR,and ANS,and the regulatory genes AN4,DPL,PHZ and MYBx were found to be downregulated under red light condition compared with their levels under white light condition.Furthermore,the light photoreceptor Cryptochrome 3(CRY3)and a series of light-dependent genes,such as PIF,HY5,andBBXs,were also determined to respond to the light treatments.The anthocyanin contents in early petunia petals under red light were significantly lower than that under white and blue light.The results of qRT-PCR further confirmed the expression pattern of some AS-related and light-response genes in response to different light quality.Yeast two-hybrid results showed that the key elements in the light signal pathway,HY5 can interact with BBX19,BBX24 and BBX25.And PHZ,the important AS regulator can induce anthocyanin synthesis in response to blue light quality fromtransient expression analysis in petunia petals.These findings presented here not only deepen our understanding of how light quality controls anthocyanin synthesis,but also allow us to explore potential target genes for improving pigment production in petunia flower petals.

关 键 词：PETUNIA ANTHOCYANIN Transcription factor Light quality Transcriptome analysis 

分 类 号：S681.9[农业科学—观赏园艺]