广西贵港地区人群MN血型等位基因多态性的调查研究  

Investigation on Allele Polymorphism of MN Blood Group in Guigang Area of Guangxi

作　　者：徐庆萍 王杰[2] 岑松光 赵卫新[1] 陆永 黄冬梅[1] 梁延连[3] XU Qing-ping;WANG Jie;CEN Song-guang;ZHAO Wei-xin;LU Yong;HUANG Dong-mei;LIANG Yan-lian(Department of Clinical Laboratory,Pingnan County People’s Hospital of Guigang City,Guangxi Pingnan 537300,China;Department of Laboratory Medicine,Dalian Medical University,Liaoning Dalian 116027,China;Institute of Transfusion Medicine,Shenzhen Blood Center,Guangdong Shenzhen 518035,China)

机构地区：[1]贵港市平南县人民医院检验科,广西平南537300 [2]大连医科大学检验医学系,辽宁大连116027 [3]深圳市血液中心输血医学研究所,广东深圳518035

出　　处：《现代检验医学杂志》2020年第6期48-51,170,共5页Journal of Modern Laboratory Medicine

基　　金：贵港市科技局立项项目(贵科转1803016);广东省医学科研基金(B2020179)。

摘　　要：目的探讨广西贵港地区人群MN血型等位基因的分布特点。方法收集2018年9月~2019年6月期间广西贵港地区随机人群300人份血样,采用血型血清学方法鉴定其MN表现型,同时提取血样中的DNA,对MN血型相关基因GYPA的exon-2序列扩增后测序,分析广西贵港地区人群MN等位基因的多态性并与中国西安、新疆、贵州、广东等地区随机人群的MN血型基因频率作比较。结果广西贵港地区人群的MN血型血清学定型与基因测序结果完全一致,300例MN血型血清学鉴定中,MM表型为140例,MN表型为121例,NN表型为39例;M基因频率为0.668,N基因频率为0.332。通过数据比较分析可知广西贵港地区人群基因各型的构成比与国内其他地区人群不同,MN血型抗原在不同个体中存在明显的剂量效应。结论广西贵港地区人群MN血型基因具有独特的多态性,了解这种多态性有助于广西贵港地区临床的安全输血,预防新生儿溶血性疾病,为广西贵港地区人类群体遗传学特征及分子生物学的研究奠定基础。Objective To investigate the distribution of MN alleles in Guigang area of Guangxi.Methods The areas random population of 300 blood samples were collected from September 2018 to June 2019 in Guangxi.Blood group serology was used to identify the MN phenotype and the DNA in the blood samples were extracted.The exon-2 sequence of MN blood group related gene GYPA were amplified and sequenced.The polymorphism of MN alleles for the population of Guigang in Guangxi were analyzed and compared with the frequency of MN blood group gene in random population with Xi’an,Xinjiang,Guizhou and Guangdong of China.Results The MN blood group were authenticated for the 300 blood samples by the serological methods and the genes were sequenced.The results showed that 140 people with MM phenotype,the 121 people with MN phenotype and the 39 people with NN phenotype were identified.The frequency of M gene was 0.668 and the N gene was 0.332.Through data comparison and analysis that can be seen the composition ratio of each genotype for the population in Guigang Area of Guangxi were different from that in other areas of China.The MN blood group antigens had obvious dose effect in different individuals.Conclusion The MN blood group genes in Guigang area of Guangxi have a unique polymorphism.Understanding this polymorphism is conduciveto the safe clinical transfusion in Guigang Area of Guangxi and the prevention of hemolytic diseases in newborns.These results lay a foundation for the study of genetic characteristicsand molecular biology of human population in Guigang Area of Guangxi.

关 键 词：MN血型 红细胞血型糖蛋白 GYPA第2外显子 MN血型基因频率 多态性 

分 类 号：R457.11[医药卫生—治疗学] Q786[医药卫生—临床医学]