儿童慢性ITP外周血CD3^(+)T细胞活性氧的检测及临床意义  

Detection and Clinical Significance of CD3^(+)T Cell Reactive Oxygen Species in Peripheral Blood of Children with Chronic ITP

作　　者：宋丽 杜宁超 李富荣 SONG Li;DU Ning-chao;LI Fu-rong(Collaborative Innovation Center of Translational Medicine,Shenzhen People’s Hospital,Guangzhou Shenzhen 510020,China;Anorectal Surgery,the Second People’s Hospital of Shenzhen,Guangzhou Shenzhen 518035,China)

机构地区：[1]深圳市人民医院转化医学协同创新中心,广州深圳518020 [2]深圳市第二人民医院肛肠外科,广州深圳518035

出　　处：《现代检验医学杂志》2020年第6期144-148,共5页Journal of Modern Laboratory Medicine

摘　　要：目的探讨儿童慢性特发性血小板减少性紫癜(Idiopathic thrombocytopenic purpura,ITP)外周血CD3^(+)T细胞中活性氧(reactive oxygen species,ROS)含量及其与血小板的关系,为临床诊断和治疗提供依据。方法随机收集30例慢性ITP患儿外周血为试验组,30例健康儿童外周血为对照组,用流式细胞术检测外周血CD3^(+)T细胞和ROS平均荧光强度,进行统计学分析。结果试验组CD3^(+)T细胞ROS平均荧光强度(15.98±5.78)明显高于对照组ROS平均荧光强度(4.65±1.03),两组间ROS的差异有统计学意义(t=2.956,P<0.05);试验组CD3^(+)T细胞ROS与血小板成负性相关关系。结论慢性ITP患儿外周血CD3^(+)T细胞的ROS明显高于对照组,说明ROS可以为儿童慢性ITP的诊断及治疗提供一定的参考依据。Objective To investigate the content of reactive oxygen species(ROS)in peripheral blood CD3^(+)T cells of children with chronic idiopathic thrombocytopenic purpura(ITP)and its relationship with platelets,so as to provide basis for clinical diagnosis and treatment.Methods Peripheral blood from 30 children with chronic ITP was randomly collected as the experimental group and 30 healthy children as the control group.The mean fluorescence intensity of CD3^(+)T cells and ROS in peripheral blood was detected by flow cytometry and analyzed statistically.Results The mean fluorescence intensity of ROS in CD3^(+)T cells in chronic ITP group(15.98±5.78)was significantly higher than that in normal control group(4.65±1.03),and the difference in ROS between the two groups was statistically significant(t=2.956,P<0.05).There was a negative correlation between ROS of CD3^(+)T cells and platelet formation in the experimental group.Conclusion The ROS of CD3+T cells in peripheral blood of children with chronic ITP was significantly higher than that of control group,indicated that ROS can provide some reference for the diagnosis and treatment of chronic ITP in children.

关 键 词：儿童 特发性血小板减少性紫癜(ITP) CD3^(+)T细胞 活性氧(ROS) 

分 类 号：R725.5[医药卫生—儿科] R392.11[医药卫生—临床医学]