静脉血真空采血管预稀释法校正EDTA依赖性假性血小板减少的效果探讨  

Discussion on the Efficacy of Prediluted Vacuum Blood Collection Tube Method to Correct EDTA-Dependent Pseudothrombocytopenia

作　　者：汪学耀 荆成宝[2] 张倩 王禅 WANG Xue-yao;JING Cheng-bao;ZHANG Qian;WANG Chan(the Third People’s Hospital of Hanbin District,Shaanxi Ankang 725018,China;Ankang Central Hospital,Shaanxi Ankang 725000,China;Ankang Hospital of Traditional Chinese Medicine,Shaaxi Ankang 725000,China)

机构地区：[1]安康市汉滨区第三人民医院,陕西安康725018 [2]安康市中心医院,陕西安康725000 [3]安康市中医医院,陕西安康725000

出　　处：《现代检验医学杂志》2020年第6期156-158,共3页Journal of Modern Laboratory Medicine

摘　　要：目的探讨静脉血真空采血管预稀释法在校正EDTA依赖性假性血小板减少症(ethylene diamine tetraacetic acid-dependent thrombocytopenia,EDTA-PTCP)的血小板(platelet,PLT)计数中的应用。方法对47例EDTA-PTCP病例血样分别使用EDTA法、静脉血真空采血管预稀释法在不同时间段测得的PLT计数进行方差分析和t检验。结果1,2和3h组内PLT计数值,EDTA法分别为(173.69±78.33)×109/L,(144.37±71.49)×109/L和(128.45±70.17)×109/L,结果差异有统计学意义(F=4.79,P=0.01);而静脉血真空采血管预稀释法分别为(206.76±71.75)×109/L,(203.35±70.31)×109/L和(190.92±75.37)×109/L,结果差异无统计学意义(F=0.65,P=0.53),稳定性高。对1,2和3h静脉血真空采血管预稀释法与EDTA法所测得的PLT计数之差进行分析,结果分别为(33.06±55.21)×109/L,(58.98±59.30)×109/L,(72.47±71.90)×109/L,差异有统计学意义(t=4.19~6.92,均P<0.001),且d>0,说明静脉血真空采血管预稀释法可以校正EDTA-PTCP的PLT计数结果。结论静脉血真空采血管预稀释法的PLT计数结果在3h内稳定性高,可以运用于EDTA-PTCP血小板检测结果的校正。Objective To investigate the application of prediluted vacuum blood collection tube in correcting platelet(PLT)count in EDTA-dependent thrombocytopenia(EDTA-PTCP).Methods Analysis of variance and t-test were performed for the PLT counts of 47 EDTA-PTCP blood samples measured by EDTA method and prediluted vacuum blood collection tube method at different time points.Results The PLT counts of the EDTA method of 1,2 and 3h groups were(173.69±78.33)×109/L,(144.37±71.49)×109/L,and(128.45±70.17)×109/L,respectively,and the results were statistically significant(F=4.79,P=0.01).Correspondingly,the results measured by the prediluted vacuum blood collection tube method were(206.76±71.75)×109/L,(203.35±70.31)×109/L,and(190.92±75.37)×109/L,respectively,and the results were not statistically significant(F=0.65,P=0.53)with high stability.The difference between the PLT counts measured by the prediluted vacuum blood collection tube method and EDTA method at 1h,2h,and 3h were analyzed.The results were(33.06±55.21)×109/L,(58.98±59.30)×109/L and(72.47±71.90)×109/L,respectively,with statistical significance(t=4.19~6.92,all P<0.001),and d>0,indicating that the prediluted vacuum blood collection tube method can correct the PLT count results of EDTA-PTCP.Conclusion The PLT count results of prediluted vacuum blood collection tube method remain stable within 3 hours and can be applied to the correction of EDTA-PTCP platelet test results.

关 键 词：EDTA依赖性假性血小板减少症 预稀释真空采血管 血小板计数 

分 类 号：R446.11[医药卫生—诊断学]