早期不同压力高压氧对中重型颅脑创伤大鼠的神经功能及IL-1β、IL-10和SOD表达的影响  

Effects of early different pressure hyperbaric oxygen on neurological function and expression of IL-1β,IL-10 and SOD in rats with moderate and severe traumatic brain injury in rats

作　　者：齐亚南 潘树义 郭大志 QI Yanan;PAN Shuyi;GUO Dazhi(Cangzhou Central Hospital,Cangzhou Hebei 061000,China;Department of Hyperbaric Oxygen,the Sixth Medical Center,Chinese PLA General Hospital,Beijing 100048,China)

机构地区：[1]沧州市中心医院,河北沧州061000 [2]中国人民解放军总医院第六医学中心高压氧科,北京100048

出　　处：《转化医学杂志》2020年第6期321-325,共5页Translational Medicine Journal

基　　金：全军医学科技青年培育计划(16QNP018);军事医学创新工程(16CXZ003);后勤科研重点项目(BHJ16J021)。

摘　　要：目的比较早期不同压力高压氧对中重型颅脑创伤大鼠的神经功能及白细胞介素(interleukin,IL)-1β、IL-10和超氧化物歧化酶(superoxide dismutase,SOD)表达的影响。方法将75只250~300 g成年雄性SD大鼠随机分为5组,分别为:①创伤性脑损伤组(traumatic brain injury,TBI组),利用PCI3000精确颅脑撞击仪采用控制性皮质撞击方法建立大鼠中重型TBI模型;②假手术组(sham组),仅将颅骨开窗,不予头部打击;③1.5绝对大气压高压氧处理组(1.5 atmosphere absolute,1.5 ATA组),在TBI组的基础上给予1.5 ATA的高压氧处理;④2.5绝对大气压高压氧处理组(2.5 ATA组),在TBI组的基础上给予2.5 ATA的高压氧处理;⑤空白对照组。所有大鼠分别在术后第1天(D1)、第3天(D3)、第7天(D7)进行颅脑损伤神经功能缺损评分(modified neurological severity score,mNSS),采用ELISA检测大鼠血清和脑组织内IL-1β、IL-10、SOD的表达情况。结果①在D1,TBI组、1.5 ATA组和2.5 ATA组大鼠mNSS评分均显著高于sham组(P<0.05);在D3和D7,2.5 ATA、1.5 ATA组mNSS评分逐渐降低,均低于TBI组(P<0.05),1.5 ATA组与2.5 ATA组无显著差异(P>0.05);②在D1,TBI组、1.5 ATA组和2.5 ATA组大鼠血清和脑组织IL-1β含量均较sham组明显升高(P<0.05);在D3和D7,1.5 ATA组和2.5 ATA组大鼠血清和脑组织IL-1β含量较TBI组均降低(P<0.05),1.5 ATA组与2.5 ATA组比较无差异(P>0.05);③在D1,TBI组、1.5 ATA组和2.5 ATA组大鼠血清和脑组织IL-10含量较sham组均升高(P<0.05);在D3和D7,1.5 ATA组和2.5 ATA组大鼠血清和脑组织IL-10含量较TBI组高(P<0.05),但1.5 ATA组大鼠的脑组织IL-10含量在D3和D7均高于2.5 ATA组,血清IL-10含量仅在D7时高于2.5 ATA组(P<0.05);④在D1和D3,TBI组、1.5 ATA组和2.5 ATA组大鼠血清和脑组织SOD含量与sham组无明显区别(P>0.05),在D7,1.5 ATA组大鼠血清和脑组织SOD含量显著高于2.5 ATA组(P<0.05)。结论早期高低不同压力高压氧治疗均可促进中重型创伤性脑损伤�Objective To compare the effects of different pressure hyperbaric oxygen on the recovery of neurological function and the expression of interleukin(IL)-1β、IL-10 and superoxide dismutase(SOD)in rats with moderate and severe craniocerebral trauma.Methods Seventy-five male adult Sprague-Dawle rats weighted 250~300 g,were randomly divided into five groups:①Traumatic brain injury group(TBI group):Rats were hit by with PCI3000 accurate brain impactor using controlled cortical impact method to establish the moderate and severe TBI model;②Sham operation group(sham group):Only open the skull,no head strike;③1.5 atmospheric absolute pressure hyperbaric oxygen treatment group(1.5 ATA group):Rats were given 1.5 ATA hyperbaric oxygen treatment after TBI;④2.5 atmospheric absolute pressure hyperbaric oxygen treatment group:Rats were given 2.5 ATA hyperbaric oxygen treatment after TBI;⑤Control group:no any treatment.All rats were evaluated by modified neurological severity score(mNSS)on day 1(D1),day 3(D3),and day 7(D7)after TBI respectively.The serum and brain tissue were also collected to detect the expression of IL-1β,IL-10 and SOD by enzyme-linked immunosorbent assay(ELISA).Results①on D1,the mNSS score in TBI group,1.5 ATA group and 2.5 ATA group was significantly higher than in sham group(P<0.05);on D3 and D7,the mNSS scores in the 2.5 ATA and 1.5 ATA groups gradually decreased and were lower than in TBI group(P<0.05),but there was no significant difference between 1.5 ATA and 2.5 ATA(P>0.05).②on D1,the level of IL-1βin serum and brain tissues in TBI,1.5 ATA and 2.5 ATA group was significantly higher than in sham group(P<0.05);on D3 and D7,the level of IL-1βin 1.5 ATA and 2.5 ATA group was lower than that in TBI group(P<0.05),but there was no difference between 1.5 ATA and 2.5 ATA group(P>0.05);③on D1,the level of IL-10 in serum and brain tissues in TBI,1.5 ATA and 2.5 ATA group was higher than in sham group(P<0.05);on D3 and D7,the level of IL-10 in serum and brain tissues in 1.5 ATA group and 2.5 ATA

关 键 词：创伤性脑损伤 高压氧 神经炎症 氧化损伤 神经损伤 

分 类 号：R651.1[医药卫生—外科学]