厦门地区9249例女性人乳头瘤病毒基因分型检测及结果分析  

Detection and result analysis of human papilloma virus genotyping in 9249 women in Xiamen area

作　　者：张玉凤[1] 雷丽云 林云[1] 罗巧[2] ZHANG Yu-feng;LEI Li-yun;LIN Yun;LUO Qiao(Department of Pathology,Xiamen Hospital of Traditional Chinese Medicine,Fujian Province,Xiamen361000,China;Department of Gynecology,Xiamen Hospital of Traditional Chinese Medicine,Fujian Province,Xiamen361000,China)

机构地区：[1]厦门市中医院病理科,福建厦门361000 [2]厦门市中医院妇科,福建厦门361000

出　　处：《中国当代医药》2020年第34期185-188,共4页China Modern Medicine

摘　　要：目的分析厦门地区妇女人乳头瘤病毒(HPV)感染的情况、各亚型的分布及其与各年龄段的关系。方法采用流式荧光杂交法对2018年7月~2019年6月在厦门市中医院妇科门诊就诊9249例女性患者的宫颈脱落细胞进行HPV基因检测,分析所有受检者HPV基因型分布情况以及单一、多重感染分布情况,并比较不同年龄组间的HPV感染特点。结果在9249例患者中,共检出HPV阳性1523例,检出率为16.47%。<20岁年龄组的检出率最高,达35.29%,其次是20~<30岁年龄组和60~<70岁年龄组,检出率分别为18.77%和18.13%。HPV感染前7位亚型依次为52、16、58、53、39、61、81型,这7种亚型检出例数占总亚型检出例数的71.24%。HPV感染以单一感染为主,占总感染的75.38%,多重感染主要是以二重感染为主,占多重感染的69.33%。结论用流式荧光杂交技术对高危人群进行HPV感染检测,不但快速准确,而且能区分HPV感染的亚型。应重视对厦门地区女性进行的HPV筛查,尤其是HPV 52、16、58、53这四种最常见亚型的筛查,能有效降低宫颈癌的发生率,以及HPV相关其他疾病的发生率。Objective To analyze the situation of human papilloma virus(HPV)infection in women in Xiamen area,and analyze the distribution of various subtypes and their relationship with different ages.Methods The flow cytometry was used to detect HPV gene in the cervical exfoliated cells of 9249 women in Clinic of Department of Gynecology of Xiamen Hospital of Traditional Chinese Medicine from July 2018 to June 2019.The distribution of HPV genotype,single and multiple infection were analyzed,and the characteristics of HPV infection among different age groups were compared.Results A total of 1523 cases were positive for HPV in 9249 cases,the detection rate was 16.47%.The highest detection rate was 35.29%in the<20 age group,followed by 18.76%and 18.13%in the 20-<30 age group and 60-<70 age group,respectively.The top 7 subtypes of HPV infection were type 52,type 16,type 58,type 53,type 39,type 61 and type 81,which accounted for 71.24%of the total.HPV infection was mainly single infection,accounting for 75.38%of the total.Multiple infection was mainly double infection,accounting for 69.33%of the multiple infection.Conclusion The detection of HPV infection in high risk population by flow cytometry is not only rapid and accurate,but also can distinguish the subtypes of HPV infection.We should pay attention to the screening of HPV in women in Xiamen area,especially the screening of the four most common subtypes of HPV 52,16,58 and 53,which can effectively reduce the incidence of cervical cancer and other HPV related diseases.

关 键 词：流式荧光杂交 人乳头瘤病毒 年龄分层 基因分型 感染 

分 类 号：R446[医药卫生—诊断学]