多重PCR检测方法在儿童流感样病例病原检测中的临床价值(附94例报告)  

Clinical value of multiple PCR detection for influenza-like cases in children: A report of 94 cases

作　　者：史大伟[1] 张艳茹 董艳[4] 葛丽霞 李丹 李静[4] 邵俊彦 郭东星 窦海伟 宛瑞杰 涂鹏 王红 辛德莉 SHI Da-wei;ZHANG Yan-ru;DONG Yan;WANG Hong(Department of Pediatrics,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China;不详)

机构地区：[1]首都医科大学附属北京友谊医院儿科,北京100050 [2]首都医科大学附属北京友谊医院北京热带医学研究所,热带病防治研究北京市重点实验室,北京100050 [3]民航总医院儿科,北京100025 [4]北京市昌平区中西医结合医院儿科,北京100096 [5]应急总医院儿科,北京100028

出　　处：《中国实用儿科杂志》2020年第11期881-884,共4页Chinese Journal of Practical Pediatrics

基　　金：国家自然科学基金面上项目(81271890)。

摘　　要：目的探讨多重聚合酶链反应(PCR)检测方法在儿童流感样病例病原检测中的临床应用价值,了解儿童流感样病例病原情况。方法收集2018年11月至2019年2月北京地区94例儿童流感样病例咽拭子标本,采用多重PCR核酸检测技术测定甲型流感病毒(InfA)、甲型流感病毒H1N1(InfA-H1N1)、甲型流感病毒H3N2(InfA-H3N2)、乙型流感病毒(InfB)、人副流感病毒、呼吸道合胞病毒(RSV)、人腺病毒(HADV)、人鼻病毒(HRV)、人博卡病毒、人偏肺病毒(HMPV)、人冠状病毒(HCoV)和肺炎支原体(MP)、衣原体13种病原核酸。结果94例标本中78例(82.98%)可测出病原,其中69例(73.40%)检出1种病原,9例(9.57%)检出2种病原;94例标本中52例(55.32%)检出流感病原,26例(27.66%)检出非流感病原。单一病原检出由多到少为InfAH1N131例,InfA-H3N210例,RSV 9例,HMPV 7例,InfA、HRV、HADV各3例,InfB、HCoV及MP各1例。9例检出2种病原的标本中,6例检出流感分别合并其他病毒(HRV、HADV、RSV、HCoV、HMPV),1例检出甲流H1N1和H3N2,2例分别检出RSV、HRV及HCoV、HADV。结论非流感病原微生物引起流感样病例并不少见,多重PCR检测有助于全面了解流感样病例的病原和混合感染情况。ObjectiveTo investigate the clinical value of multiple PCR method in detecting pathogens in influenza-like cases in children and to understand the distribution of respiratory pathogens in pediatric influenza-like cases.Methods A total of 94 throat swabs of childhood flu-like cases admitted from November 2018 to February 2019 in Beijing area were collected.Multiple PCR method was used to detect influenza A virus,influenza A virus-H1N1,influenza A virus-H3N2,influenza B,parainfluenza virus,RSV,human adenovirus,human rhinovirus,human bocavirus,human metapneumovirus,human coronavirus,mycoplasma pneumonia and chlamydia gene.ResultsOf the 94 specimens,78(82.98%)were detected pathogens,of which one pathogen was detected in 69 cases(73.40%),and 2 pathogens in 9 cases(9.57%).Fifty-two(55.32%)of 94 specimens were detected influenza pathogens and 26 cases(27.66%)detected non-flu pathogens.The number of single pathogens detectedwas 31 cases of Inf A-H1N1,10 cases of Inf A-H3N2,9 cases of RSV,7 cases of HMPV,3 cases of Inf A,HRV and HADV each,and 1 case of Inf B,HCo V and MP each.Of the 9 specimens with 2 pathogens detected,6 were with influenza combined with other viruses(HRV,HADV,RSV,HCo V,HMPV),1 with H1N1 and H3N2,and 2 with RSV,HRV and HCo V,HADV,respectively.ConclusionInfluenzalike cases caused by non-influenza pathogenic microorganisms are common.Multiple PCR detection can help to understand the pathogen distribution and mixed infection in influenza-like cases.

关 键 词：多重PCR检测 流感样病例 呼吸道病原 儿童 

分 类 号：R72[医药卫生—儿科]