地黄甙D对四氯化碳诱导的肝纤维化大鼠的保护作用  

Protective Effect of Rehmainonoside D on Hepatic Fibrosis Induced by Carbon Tetrachloride in Rats

作　　者：何华 杨弋[2] 陈丹镝[1] HE Hua;YANG Yi;CHEN Dandi(West Chinese School of Public Health and West China Forth Hospital,Sichuan University,Chengdu,610041,China;West China Second Hospital of Sichuan University,Chengdu,610044,China)

机构地区：[1]四川大学华西公共卫生学院/四川大学华西第四医院,成都市610041 [2]四川大学华西第二医院,成都市610044

出　　处：《医学分子生物学杂志》2020年第6期448-452,共5页Journal of Medical Molecular Biology

基　　金：四川省科学技术厅应用基础项目(No.2019YJO02O)。

摘　　要：目的探究地黄甙D对四氯化碳诱导的肝纤维化大鼠的保护作用。方法40只SD大鼠随机分成4组:对照组、模型组、地黄甙D20 mg/kg组和地黄甙D60 mg/kg组,每组10只。采用腹腔注射四氯化碳法构建肝纤维化大鼠模型。采用酶联免疫吸附法检测血天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(CⅣ)水平;蛋白质免疫印迹法检测肝组织中天冬氨酸特异性半胱氨酸蛋白酶3(Caspase-3)水平结果与模型组比较,地黄甙D组大鼠AST、ALT、HA、LN、PCⅢ、CⅣ及Caspase-3水平均降低,差异均有统计学意义(P<0.05)。地黄甙D60 mg/k g组大鼠AST、ALT、H A、LN、PCⅢ、CⅣ及Caspase-3水平均低于地黄甙D20 mg/kg组,差异均有统计学意义(P<0.05)。结论地黄甙D对四氯化碳诱导的肝纤维化大鼠有一定保护作用,其作用可能与调控Caspase-3表达有关。Objective To explore the protective effect of rehmainonoside D on carbon tetrachloride-induced liver fibrosis in rats.Methods Forty SD rats were randomly divided into four groups:control group,model group,20 mg/kg rehmainonoside D group and 60 mg/kg rehmainonoside D group,with 10 rats in each group.A rat model of liver fibrosis was established by intraper-itoneal injection of carbon tetrachloride.Blood aspartate aminotransferase(AST),alanine aminotransferase(ALT),hyaluronic acid(HA),laminin(LN),type Ⅲ procollagen(PC Ⅲ),type Ⅳ collagen(C Ⅳ)levels were detected by enzyme-linked immunosorbent assay.Western blotting was used to detect Caspase-3 levels in liver tissues.Results The levels of AST,ALT,HA,LIN,PC Ⅲ,C Ⅳ and Caspase-3 were significantly decreased in rats in the rehmainonoside D groups when compared with those in the model group,and the differences were statistically significant(P<0.05).Those indicators were also much lower in the 60 mg/kg rehmainonoside D group than those in the 20 mg/kg Rehmainonoside D group,with the differences being statistically significant(P<0.05).Conclusion Rehmainonoside D has a protective effect on carbon tetrachloride-induced liver fibrosis in rats,and its effect may be related to the regulation of Caspase-3 expression.

关 键 词：肝纤维化 大鼠 地黄甙D 四氯化碳 

分 类 号：R965[医药卫生—药理学] R575.2[医药卫生—药学]