微污染物对饮用水管网中微生物再生长的影响  

Effect of Micro-pollutants on Bacterial Regrowth in Drinking Water Pipelines

作　　者：沈怡 王海波[2] 胡春 赵丹[1] SHEN Yi;WANG Hai-bo;HU Chun;ZHAO Dan(School of Environmental Science and Engineering,Suzhou University of Science and Technology,Suzhou 215011,China;Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China;Institute of Environmental Research at Greater Bay,Guangzhou University,Guangzhou 510006,China)

机构地区：[1]苏州科技大学环境科学与工程学院,江苏苏州215011 [2]中国科学院生态环境研究中心,北京100085 [3]广州大学大湾区环境研究院,广东广州510006

出　　处：《中国给水排水》2020年第21期15-20,共6页China Water & Wastewater

基　　金：国家自然科学基金资助项目(51878654、51838005);中国科学院重点前沿课题(QYZDY-SSW-DQC004)。

摘　　要：通过对自来水厂砂滤出水添加磺胺嘧啶和环丙沙星的方法,研究了不同微污染物对管网出水自由悬浮的微生物包括致病菌再生长的影响。首先通过定量PCR分析发现,微污染物的存在降低了分枝杆菌属、军团菌属、嗜肺军团菌和铜绿假单胞菌的含量。但是,总细菌16S rRNA、棘阿米巴变形虫、哈氏虫和鸟分枝杆菌含量增加,尤其是复合污染条件下这些微生物的增加更明显。另外,通过对不同管网出水微生物分泌的胞外多聚物(EPS)以及抗药基因进行分析发现,EPS中多糖和蛋白质及外排泵基因mexA、整合子基因int1和抗磺胺嘧啶基因sul1、sul2、sul3是导致微生物增加的主要原因。因此,为了更好地控制管网水质,应进一步采取有效措施去除水源水中的微污染物。Effect of different micro-pollutants on the regrowth of free-living bacteria including pathogens was studied,based on the method of adding sulfadiazine and ciprofloxacin to the effluent of sand filtration in water treatment plant.Firstly,the addition of micro-pollutants decreased the amounts of Mycobacterium spp.,Legionella spp.,Legionella pneumophila and Pseudomonas aeruginosa.However,the amounts of 16S rRNA,Acanthamoeba spp.,Hartmanella vermiformis and Mycobacterium avium increased.The amounts of these bacteria increased obviously,especially when sulfadiazine and ciprofloxacin were added to the water simultaneously.Moreover,the extracellular polymeric substances(EPS)and antibiotic resistance genes(ARGs)in the effluent of drinking water pipelines were also studied.The results indicated that the polysaccharide and protein in the EPS and ARGs including mexA,int1,sul1,sul2 and sul3 were the main factors for the increase of the bacterial amounts in the effluent.In order to effectively control the water quality of the pipelines,further measures should be conducted to remove the micro-pollutants in the source water.

关 键 词：微污染物 微生物再生长 条件致病菌 胞外多聚物 抗药基因 

分 类 号：TU991[建筑科学—市政工程]