产葡萄糖异构酶菌株的筛选及酶学性质研究  

Screening of glucose isomerase-producing strains and study of their enzymatic properties

作　　者：廖贵芹 程国军[2] 吴和涛 滕雪 刘莹[2] LIAO Guiqin;CHENG Guojun;WU Hetao;TENG Xue;LIU Ying(College of Life Science & Technology, Huazhong University of Science and Technology, Wuhan 430074, China;College of Life Sciences, South-Central University for Nationalities, Wuhan 430074, China)

机构地区：[1]华中科技大学生命科学与技术学院,武汉430074 [2]中南民族大学生命科学学院,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2020年第6期580-585,共6页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：湖北省自然科学基金资助项目(2016CFC771);中南民族大学实验室开放项目(KF2017007)。

摘　　要：以土壤为材料,经过初筛平板富集培养,分离纯化得到了能正常生长的28株细菌和35株放线菌;再分别进行液体发酵,用改进的硫酸-咔唑法测定胞内、外酶活,筛选出了一株产胞外葡萄糖异构酶活力最高的细菌株菌XJ-27.对该菌株进行形态特征和16S rDNA序列比对分析,并对其胞外葡萄糖异构酶进行了部分性质研究.结果表明:该菌株酶活力为242.1 U·mL^-1,属于芽孢杆菌属(Bacillus),70℃测得最适pH为7.0~7.8;在pH 7.4时其最适反应温度为70~80℃;Mg^2+和Co^2+对其酶活力有显著提高,Mg^2+的效果最明显.After initial screening and on-plate enrichment culture,28 strains of bacteria and 35 strains of actinomycetes were isolated and purified from multiple soil samples.Liquid fermentation was carried out separately,the intracellular and extracellular enzyme activities were determined by improved carbazole-sulfate method.XJ-27,a bacterial strain with the highest activity of extracellular glucose isomerase was selected.XJ-27 was analyzed by morphological characterization and 16S rDNA sequence.Some properties of extracellular glucose isomerase of this strain were also studied.The results indicated that XJ-27 belonged to Bacillus,and its enzyme activity was 242.1 U·mL^-1.The optimum pH was 7.0-7.8,and the optimum reaction temperature was 70-80℃under pH 7.4.Mg^2+and Co^2+could significantly increase the enzyme activity and Mg^2+was the most effective.

关 键 词：葡萄糖异构酶 高果糖浆 液体发酵 果糖含量测定 酶活测定 

分 类 号：Q933[生物学—微生物学]