稀土配位聚合物荧光探针的制备及其对痕量汞离子的检测  

Preparation of a Fluorescent Probe Based on Terbium Coordination Polymers and Its Detection on Trace Mercury Ions

作　　者：张磊[1] 许森 赵雅梦 陆鸿宇 于博昊[1] 张维冰[1] ZHANG Lei;XU Sen;ZHAO Ya-meng;LU Hong-yu;YU Bo-hao;ZHANG Wei-bing(School of Chemistry & Molecular Engineering,East China University of Science and Technology,Shanghai 200237,China)

机构地区：[1]华东理工大学化学与分子工程学院,上海200237

出　　处：《分析测试学报》2020年第12期1527-1532,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金(21707034);中央高校基本科研业务费(222201814020);上海市科委仪器试剂专项(18142202500);教师产学研践习计划(YJ0130538)。

摘　　要：以稀土金属铽离子为发光中心离子,鸟苷酸(GMP)为配体制备得到了能够发射绿色荧光的稀土配位聚合物。并通过加入小牛胸腺DNA溶液进一步增强荧光强度,形成“turn-on”荧光类型的稀土配合物,利用汞离子与小牛胸腺DNA的结合以及与配体GMP的相互作用使荧光猝灭,形成“turn-on-off”类型稀土荧光探针,实现了汞离子的检测。Hg2+质量浓度在50~1200μg·L^-1范围内与体系荧光强度变化程度呈线性关系(I0-I)/I0=0.0002C+0.0107,相关系数(r2)为0.9915,检出限为1.5μg·L^-1,相对标准偏差(RSD)为2.3%。对河水中Hg2+进行加标回收检测,回收率为98.1%~99.8%,RSD为0.28%~1.9%。A“turn-on-off”type fluorescent probe based on terbium coordination polymers was prepared for the detection of trace mercury ions.The terbium coordination polymers were prepared with terbium ions as luminescence center and guanyl-5-disodium phosphate(GMP)as ligand,which could emit green fluorescence.The intensity of the luminescence was increased with the addition of ct-DNA,and a“turn-on”type of fluorescent probe was formed.Then,the“turn-on-off”type fluorescent probe was formed by quenching the fluorescence with the interaction between the heavy metal mercury ions and ct-DNA and ligand of GMP with the addition of mercury ions.The calibration curve for mercury ions was linear in the range of 50-1200μg·L^-1,with a correlation coefficient(r2)of 0.9915 and a regression equation of(I0-I)/I0=0.0002 C+0.0107.The limit of detection was 1.5μg·L^-1,while the relative standard deviation(RSD)was 2.3%(C=600μg·L^-1,n=5).The probe was applied to the detection of trace mercury ions in river water with recoveries of 98.1%-99.8%and RSD of 0.28%-1.9%.

关 键 词：稀土配位聚合物 CT-DNA 铽离子 荧光光谱法 汞离子 

分 类 号：O657.3[理学—分析化学] O614.243[理学—化学]