山竹果皮提取物“芒果醚”抑制乳腺癌细胞MCF-7增殖与迁移  

Mangosteen peel extract"Mango Ether"inhibits proliferation and migration of breast cancer cell MCF-7

作　　者：黄凯悦 刘敏燕 陈婷[1] 陆昌瑞 赵越 邵志宇[1] 张云龙[1] HUANG Kaiyue;LIU Minyan;CHEN Ting;LU Changrui;ZHAO Yue;SHAO Zhiyu;ZHANG Yunlong(College of Chemistry Chemical Engineering and Bioengineering,Donghua University,Shanghai 201620,China)

机构地区：[1]东华大学化学化工与生物工程学院,上海201620

出　　处：《生物学杂志》2020年第6期38-41,共4页Journal of Biology

基　　金：国家自然科学基金(No.81671508,No.31000352);上海市科委“一带一路”国际合作项目(19410741800);校企合作项目(No.HX105180781);上海市大学生创新创业计划项目(No.105-03-0178139,105-03-0178229)。

摘　　要：从山竹(Garcinia mangostana)果皮内提取和鉴定了一种黄酮类化合物“芒果醚”,虽已证实其具有抑瘤作用,但机制不清。为了检测此芒果醚对乳腺癌细胞增殖抑制效果,以正常人乳腺细胞MCF-10作为对照,探析了其对MCF-7、SUM1315MO2和MDA-MB-2313种乳腺癌细胞增殖的影响,证实其对MCF-7细胞具有剂量和时间依赖性抑制作用,24 h给药处理的IC 50值为20μmol/L,同时出现凋亡小体。蛋白质免疫印迹反应检测凋亡信号途径显示:Bcl-2剂量依赖性下调,而Bax上调,Cytochrome C显著上调,Precaspase-9也明显下调,这表明芒果醚通过促发线粒体依赖性凋亡途径而抑制了MCF-7增殖。另外,划痕实验检测结果显示,芒果醚可有效减弱MCF-7细胞迁移。山竹果皮提取物“芒果醚”具有抑制乳腺癌细胞MCF-7增殖与迁移作用,为其用于抗癌治疗开发提供了相关依据。A kind of flavonoid"Mango Ether"was extracted and identified from mangosteen peel,and its anti-tumor activities in breast cancer cells were proved.However,the mechanism of Mango Ether is unclear.Three kinds of breast cancer cells,MCF-7,SUM1315MO2 and MDA-MB-231 were used to detect anti-proliferation effects of mango ether,taken the normal human breast cell MCF-10 as control cells.As results,Mango Ether had anti-proliferation effect on MCF-7 cells in a dose-and time-dependent manner.Its IC 50 value of 24 h was 20μmol/L,accompanied with apoptotic bodies.The detection of apoptotic signaling pathway by Western Blot showed that the expression of Bcl-2 down-regulated in a dose-dependent manner,while Bax and Cytochrome C significantly up-regulated,as well as the precaspase-9 expression also down-regulated.These results indicated that Mango Ether inhibited MCF-7 cells proliferation by a mitochondria-dependent apoptotic pathway.In addition,the results of the scratch test showed that Mango Ether can effectively attenuate the migration of MCF-7 cells.In conclusion,mangosteen peel extract,Mango Ether,could inhibit the proliferation and migration of breast cancer MCF-7 cells,which provids useful evidence for anti-tumor treatment strategy.

关 键 词：芒果醚 乳腺癌 细胞增殖 细胞凋亡 细胞迁移 

分 类 号：R931[医药卫生—生药学]