西藏濒危植物大花黄牡丹内生真菌及其根际土壤真菌的群落多样性研究  

Diversity of endophytic fungi and the rhizosphere soil fungi communities of the endangered plant Paeonia ludlowii in Tibet

作　　者：禄亚洲 张二豪[2,3] 尹秀 蔡皓[2,3] 袁雷 李连强 赵垦田[4] 兰小中 LU Yazhou;ZHANG Erhao;YIN Xiu;CAI Hao;YUAN Lei;LI Lianqiang;ZHAO Kentian;LAN Xiaozhong(Institute of Plateau Ecology,Tibet Agricultural and Animal Husbandry College,Nyingchi 860000,China;Tibet Agricultural and Animal Husbandry College-Southwest University Medicinal Plants Joint Research and Development Centre,Nyingchi 860000,China;Food Science School,Tibet Agricultural and Animal Husbandry College,Nyingchi 860000,China;School of Resources and Environment,Tibet Agriculture and Animal Husbandry College,Nyingchi 860000,China)

机构地区：[1]西藏农牧学院高原生态研究所,林芝860000 [2]西藏农牧学院西南大学药用植物联合研发中心,林芝860000 [3]西藏农牧学院食品科学学院,林芝860000 [4]西藏农牧学院资源与环境学院,林芝860000

出　　处：《生物学杂志》2020年第6期59-63,共5页Journal of Biology

基　　金：西藏自治区科学技术厅科学研究一般项目(XZ2017ZRG-22);西藏自治区科技计划项目科技重大专项(XZ201901-GA-04)。

摘　　要：以西藏特有濒危藏药材大花黄牡丹为材料,利用高通量测序技术对大花黄牡丹不同组织内生真菌及根际土壤真菌的ITS(Internal transcribed spacer)序列进行测序和分析。结果表明:所有样品中共获得真菌OTUs(Operational taxonomic units)796个,其中,根部228个,茎部55个,叶部70个,花部110个,果实69个,上、中、下根际土壤分别为421、319和258个,隶属8门13纲27目50科67属。大花黄牡丹不同植物组织中共有5个OTUs,不同深度根际土壤共有135个OTUs。Biomarker分析表明在植物组织中的标志类群是Mycosphaerellaceae和Cladosporiaceae,土壤中的标志性类群是Nectriaceae。不同大花黄牡丹组织内生真菌群落丰富度和多样性不同,其根部内生真菌物种最丰富。不同深度根际土壤中真菌具有相似的丰富度与多样性,根部和根际土壤中真菌群落组成相似。植物组织及根际土壤中的标志类群可能具有药用功能,为大花黄牡丹内生真菌和根际真菌的进一步研究奠定了基础。The endemic and endangered plant P.ludlowii was used as the material,high-throughput sequencing technique was used to sequence and analyze the Internal transcribed spacer(ITS)of endophytic fungi and rhizosphere soil fungi in P.ludlowii.The results showed that a total of 796 Operational taxonomic units(OTUs)were obtained in all samples.Among them,228 OTUs were from the root,55 OTUs from the stem,70 OTUs from the leaf,110 OTUs from the flower,69 OTUs from the fruit.The number of upper,middle,and lower rhizosphere soils respectively were 421,319,and 258 OTUs,belonging to 8 phylum,13 class,27 order,50 family and 67 genus.The different tissues shared 5 OTUs,and 135 OTUs were shared in different soil samples.Biomarker analysis showed that Mycosphaerellaceae and Cladosporiaceae were mark groups in different plant tissues,and Nectriaceae was mark group in rhizosphere soil.The abundance and diversity of endophytic fungal communities in different tissues of P.ludlowii were different,and the root had the most diverse species of endophytic fungi.The richness and diversity of different fungi in rhizosphere soils were similar.Representative species might have medicinal function in plant tissues and rhizosphere soils,and fungal communities in root and rhizosphere soils were similar.The result of this study provided on basis for further research on endophytic and rhizosphere fungi of P.ludlowii.

关 键 词：大花黄牡丹 真菌 群落多样性 高通量测序 

分 类 号：Q939.96[生物学—微生物学] S685.11[农业科学—观赏园艺]