基质辅助激光解吸电离飞行时间质谱技术在肿瘤诊断及微生物鉴定中的应用  

Application of matrix assisted laser desorption ionization time of flight mass spectrometry in tumor diagnosis and microbial identification

作　　者：管明秀 张文成[2] 王宝占[1] 崔喆 陈宝颖 张霞[3] GUAN Ming-xiu;ZHANG Wen-cheng;WANG Bao-zhan(Department of Clinical Laboratory,Baodi College of Clinical Medicine,Tianjin Medical University,Tianjin 301800,China)

机构地区：[1]天津医科大学宝坻临床学院检验科,天津301800 [2]天津医科大学宝坻临床学院肿瘤科,天津301800 [3]天津医科大学宝坻临床学院心内科,天津301800

出　　处：《实用医药杂志》2020年第12期1133-1137,共5页Practical Journal of Medicine & Pharmacy

摘　　要：基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption ionization time-of-flight mass spectrometry,MALDI-TOF MS)技术具有操作简单、快速、高通量、高灵敏度和高准确度的特点,特别适合于蛋白、多肽等生物大分子的高通量筛选,也可用于核酸的快速分析。笔者就MALDI-TOF MS对癌症的诊断和微生物的鉴定进行综述。Matrix assisted laser desorption time of flight mass spectrometry(MALDI-TOF MS)is characterized by simple operation,high throughput,high sensitivity and high accuracy.It is especially suitable for high-throughput screening of proteins,peptides and other biological macromolecules,and also for rapid analysis of nucleic acids.This paper reviews the diagnosis of cancer and the identification of microorganism by MALDI-TOF MS.

关 键 词：光谱法 基质辅助激光解吸电离 蛋白质组学 癌症 微生物鉴定 

分 类 号：R446.5[医药卫生—诊断学]