不同培养条件下菌株NB5对氨氮的去除效果研究  

Removal effect of strain NB5 on ammonia nitrogen under different aquaculture conditions

作　　者：胡晓娟[1,2] 文国樑 田雅洁[1] 苏浩昌 徐武杰[1,2] 徐煜 许云娜[1] 曹煜成 HU Xiaojuan;WEN Guoliang;TIAN Yajie;SU Haochang;XU Wujie;XU Yu;XU Yunna;CAO Yucheng(South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation&Utilization,Ministry of Agriculture and Rural Affairs/Guangdong Provincal Key Laboratory of Fishery Ecology and Environment,Guangzhou 510300,China;Shenzhen Base of South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shenzhen 518121,China)

机构地区：[1]中国水产科学研究院南海水产研究所/农业农村部南海渔业资源开发利用重点实验室/广东省渔业生态环境重点实验室,广东广州510300 [2]中国水产科学研究院南海水产研究所深圳试验基地,广东深圳518121

出　　处：《南方水产科学》2020年第6期89-96,共8页South China Fisheries Science

基　　金：中国水产科学研究院基本科研业务费专项资金(2020TD54);国家重点研发计划“蓝色粮仓科技创新”专项(2019YFD0900402);中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助项目(2019TS06);现代农业产业技术体系建设专项资金(CARS-48);广东省促进经济发展专项资金(现代渔业发展用途)(粤农2019B12)。

摘　　要：文章研究了从硝化菌群中分离的菌株NB5在不同培养条件下的生长情况及其对氨氮(NH4+-N)和亚硝氮(NO2−-N)的去除效果,并对菌株NB5进行了鉴定。结果发现,菌株NB5在盐度25~45、pH 6.0~9.0、15~35℃和通气量1~2 L·min^−1的条件下生长良好(P>0.05)。在盐度45、35和25条件下对NH4+-N的最大去除率分别为96.24%、88.93%和75.08%;pH 7.5和9.0条件下分别为99.53%和99.37%;温度30、25、15和35℃条件下分别为99.53%、97.22%、97.29%和71.26%;通气量为2和1 L·min^−1时分别为99.87%和99.82%。在上述培养条件下菌株NB5对NH4+-N的最大去除率均显著高于对照组和其他条件组(P<0.05)。菌株NB5在不同培养条件下对NO2−-N浓度变化无显著作用(P>0.05)。经16S rDNA序列分析,菌株NB5鉴定为海水硝酸盐还原菌(Nitratireductor aquimarinus)。研究表明,菌株NB5具有较好的环境适应性和氨氮去除效果,尤其适合中高盐度(25~45)养殖池塘环境。We studied the growth of strain NB5 isolated from nitrifying bacteria and its removal effect on ammonia nitrogen(NH4+-N)and nitrite nitrogen(NO2−-N)under different aquaculture conditions,and identified this bacterium.The results show that strain NB5 grew well at salinity of 25−45,pH of 6.0−9.0,temperature of 15−35℃and ventilations of 1−2 L·min^−1(P>0.05).At salinities of 45,35 and 25,the maximum removal rates of NH4+-N were 96.24%,88.93%and 75.08%,respectively.At pHs of 7.5 and 9.0,they were 99.53%and 99.37%,respectively.At temperatures of 30,25,15 and 35℃,they were 99.53%,97.22%,97.29%and 71.26%,respectively.At ventilations of 2 and 1 L·min^−1,they were 99.87%and 99.82%,respectively.Under the above conditions,the maximum removal rates of NH4+-N were significantly higher than those of the control group and the other groups(P<0.05).Strain NB5 had no significant effect on NO2−-N concentration under different aquaculture conditions(P>0.05).By 16S rDNA sequence analysis,strain NB5 was identified as Nitratireductor aquimarinus.The results indicate that strain NB5 has good environmental adaptability,especially suitable for aquaculture pond environment at medium and high salinities(25−45).

关 键 词：海水硝酸盐还原菌 氨氮 培养条件 

分 类 号：Q178.1[生物学—水生生物学]