低龄儿童龋病牙菌斑的菌群结构分析  

Structure of bacteria in dental plaque in children with early childhood caries

作　　者：张侬[1] 李高驰[2] 蒋俊豪[1] 周潜 温丽娟[2] 周乐天 张玲[1] 杨雅[1] 魏凤香[2] ZHANG Nong;LI Gaochi;JIANG Junhao;ZHOU Qian;WEN Lijuan;ZHOU Letian;ZHANG Ling;YANG Ya;WEI Fengxiang(Department of Stomatology,Shenzhen Longgang District Maternity&Child Healthcare Hospital,Shenzhen,Guangdong 518172,China;不详)

机构地区：[1]深圳市龙岗区妇幼保健院口腔科,广东深圳518172 [2]深圳市龙岗区妇幼保健院中心实验室,广东深圳518172 [3]深圳市微健康基因研究院,广东深圳518129

出　　处：《中国微生态学杂志》2020年第11期1279-1284,共6页Chinese Journal of Microecology

基　　金：深圳市龙岗区经济与科技发展专项资金——医疗卫生科技计划项目资助(LKKCYLWS2018000187)。

摘　　要：目的通过低龄龋齿儿童与口腔健康儿童的微生物组检测,探讨低龄儿童龋病牙菌斑的菌群组成特征。方法在深圳地区招募轻度龋齿、重度龋齿及口腔健康儿童共100名,采集其牙菌斑或健康牙齿表面微生物样本,采用MiSeq测序平台进行16S V4-V5区域的高通量测序。同时,通过生物信息分析方法,对低龄龋齿儿童的牙菌斑的微生物组成的特征进行挖掘分析。结果相对口腔健康儿童,重度龋齿患儿的牙菌斑微生物多样性增加,轻度龋齿患儿则减少,但变化不显著。门水平的优势菌主要为放线菌门、变形菌门及拟杆菌门,虽有相对丰度变化但差异无统计学意义。前10位的优势菌属中,轻度及重度龋齿儿童的最高丰度优势菌属为棒状杆菌属,重度龋齿儿童与健康儿童中的棒状杆菌丰度差异有统计学意义(t=-2.1955,P=0.0281)。健康儿童口腔的优势菌属包含了卟啉单胞菌属,但在轻度及重度龋齿儿童的优势菌属则替换为月形单胞菌属。种水平的菌群结构分析显示,前10位的优势菌丰度变化差异无统计学意义,而低丰度的致病菌如变异链球菌(H=27.3021,P<0.0001)、Atopobium parvulum(H=17.4182,P=0.0002)、栖牙普氏菌(H=10.5989,P=0.0050)、唾液普氏菌(H=10.0350,P=0.0066)等则在重度龋齿儿童中显著富集。结论低龄龋齿儿童的牙菌斑微生物结构发生了改变,部分口腔有害菌的丰度显著增加与龋病严重程度密切相关。Objective To observe the bacterial composition of dental plaque in children with early childhood caries(ECCs)vs healthy children through microbial sequencing analysis.Methods A total of 100 children at ages of 3-6 years in Shenzhen,including children with ECCs or severe early childhood caries(SECCs)and healthy children,were enrolled.Microbial samples from dental plaque or healthy teeth surface were collected.The V4-V5 region of 16 s DNA gene was sequenced using MiSeq platform,and the characteristics of the microbial composition were analyzed with microbial bioinformatics method.Results Compared with the healthy children,there was no significant change in the microbial diversity in children with caries.At the phylum level,the dominant bacteria were Actinomycetes,Proteobacteria and Bacteroidetes.Among the top 10 predominant bacteria genera,Corynebacterium had the highest abundance in children with ECCs and SECCs,and it significantly enriched in children with SECCs compared to that in healthy children(t=-2.1955,P=0.0281).Porphyromonas was dominant bacteria in the oral cavities of healthy children,which was replaced by Selenomonas in children with ECCs and SECCs.The abundance of the top 10 dominant species didn′t significantly change.On the other hand,low abundant Streptococcus mutans(H=27.3021,P<0.0001),Atopobium parvulum(H=17.4182,P=0.0002),Prevotella denticola(H=10.5989,P=0.0050)and Prevotella salivae(H=10.0350,P=0.0066)enriched significantly in children with SECCs.Conclusion The composition of microbes in dental plaque changed in children with ECCs,especially that of some significantly enriched pathogens,which was strongly associated with the severity of dental caries.

关 键 词：低龄儿童 龋齿 牙菌斑 菌群 高通量测序 

分 类 号：R378[医药卫生—病原生物学]