上海市金山区人杯状病毒感染聚集性疫情的流行病学及病毒基因特征分析  

Epidemiological and genetic analysis of human calicivirus in cluster epidemic situation in Jinshan District,Shanghai

作　　者：宋灿磊[1] 董兆鹏 杜荐如 朱琳 王唐[1] 李淑华[1] SONG Can-lei;DONG Zhao-peng;DU Jian-ru;ZHU Lin;WANG Tang;LI Shu-hua(Jinshan Municipal Center for Disease Control and Prevention,Shanghai 201599,China)

机构地区：[1]上海市金山区疾病预防控制中心,上海201599

出　　处：《中国卫生检验杂志》2020年第22期2701-2704,共4页Chinese Journal of Health Laboratory Technology

基　　金：上海市卫生健康委面上项目(201940428);金山区医学重点专科(传染病流行病学)建设项目(JSZK2015B08)。

摘　　要：目的分析上海市金山区人杯状病毒感染聚集性疫情的流行病学和毒株基因特征,为制定防控策略提供科学依据。方法运用描述性分析方法对2018年-2019年4月金山区人杯状病毒感染聚集性疫情及个案资料开展分析,每起疫情随机选取经RT-PCR检测呈阳性的核酸的PCR产物进行序列测定和基因特征分析。结果共报告8起聚集性杯状病毒感染性疫情,病例115例,总罹患率为20.14%;疫情持续2 d^9 d。采集103份样本中,诺如病毒及札如病毒核酸阳性59份,检出率为57.28%。基因序列分析证实,诺如病毒GⅡ.3型2起、GⅡ.4Sydney-2012型、诺如病毒GⅡ.2型、GⅡ.6型、札如病毒GⅠ.1型、诺如病毒GⅡ.2型伴札如病毒GⅠ.3型感染、GⅡ.4Sydney-2012型伴GⅡ.17型混合感染各1起。结论金山区人杯状病毒感染聚集性胃肠炎疫情冬春季多发,GⅡ组诺如病毒为优势毒株,基因呈现多样性,应重点加强幼托机构、学校等重点场所人杯状病毒感染性疫情防控。Objective To analyze the epidemiological and genetic analysis of human calicivirus in cluster epidemic situation in Jinshan District,Shanghai. Methods Descriptive analysis was used to analyze the clustered epidemic situation and case data of human calicivirus infection in Jinshan District from 2018 to April 2019. PCR products of nucleic acids positive by RT-PCR were randomly selected for sequence determination and gene characterization analysis in each outbreak. Results A total of 8 cases of aggregate calicivirus infection were reported,including 115 cases,with a total incidence rate of 20. 14%. The outbreak lasted from 2 to 9 days. Among 103 samples,59 were positive for norovirus and sappoviruses nucleic acid,with a detection rate of 57. 28%. Gene sequence analysis confirmed that there were two norovirus G Ⅱ. Type 3 infection,one G Ⅱ. 4 Sydney-2012 type infection,one norovirus G Ⅱ. type 2 infection,one G Ⅱ. type 6 infection,one sappoviruses G Ⅰ. type 1 infection,one norovirus G Ⅱ. type 2 infection with sappoviruses G Ⅰ type 3 infection,and G Ⅱ. 4 Sydney-2012 with G Ⅱ. 17 infection. Conclusion Human calicivirus was mainly the pathogen associated with the outbreak of vomit and diarrhea in Jinshan District,GⅡ norovirus is the dominant strain with genetic diversity and high incidence in winter and spring. Therefore,the prevention and control of norovirus infection in schools and childcare institutions should be emphasized.

关 键 词：人杯状病毒 诺如病毒 札如病毒 暴发 流行病学 

分 类 号：R725.1[医药卫生—儿科]