沈阳市2012年-2018年食源性致病菌监测金黄色葡萄球菌多位点可变数量串联重复序列分析分型  

Monitoring of Staphylococcus aureus MLVA subtyping from 2012 to 2018 in Shenyang City

作　　者：连英姿[1] 侯元[1] 郝富智 赵常智[1] 刘虎生[1] 魏夺[1] LIAN Ying-zi;HOU Yuan;HAO Fu-zhi;ZHAO Chang-zhi;LIU Hu-sheng;WEI Duo(Shenyang Health Service and Administrative Law Enforcement Center,Shenyang Municipal Center for Disease Control and Prevention,Shenyang,Liaoning 110031,China)

机构地区：[1]沈阳市卫生健康服务与行政执法中心沈阳市疾病预防控制中心,辽宁沈阳110031

出　　处：《中国卫生检验杂志》2020年第22期2810-2812,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的建立金黄色葡萄球菌监测的多位点可变数量串联重复序列分析(MLVA)分子分型数据库,提高金黄色葡萄球菌的检测和防控能力。方法筛选针对沈阳本地食源性致病菌具有高度多态性的VNTR位点,STTR1、STTR2、STTR3、STTR5、STTR6、STTR7、STTR8、STTR9共8个VNTR位点进行MLVA分型。结果186份样品检出金黄色葡萄球菌20株,检出率为10.8%。20株金黄色葡萄球菌共分成A和B两大聚类,分别占比65%和35%,A聚类可分为3个亚聚类,B聚类可分为2个亚聚类。表明沈阳地区流行的金黄色葡萄球菌是多克隆的,大部分菌株属于一个亲缘关系很近的克隆系。结论沈阳地区金黄色葡萄球菌MLVA分型呈较好的多态性。Objective To establish a multisite variable number series repeated sequence analysis( MLVA) database for Staphylococcus aureus monitoring to improve the detection and control ability of Staphylococcus aureus. Methods A total of 8 VNTR sites with high polymorphism were selected in Shenyang,including STTR1,STTR2,STTR3,STTR5,STTR6,STTR7,STTR8 and STTR9. Results Twenty Staphylococcus aureus strains were detected in 186 samples with a detection rate of 10. 8%. The20 strains of Staphylococcus aureus are divided into two major clusters,A and B,respectively accounting for 65% and 35%.The A cluster can be divided into 3 sub-clusters,and the B cluster can be divided into 2 sub-clusters. It is indicated that Staphylococcus aureus is polyclonal in Shenyang area,and most strains belong to a closely related clone line. Conclusion The classification of Staphylococcus aureus MLVA typing in Shenyang area showed good polymorphism.

关 键 词：金黄色葡萄球菌 多位点可变数量串联重复序列分析 分型 

分 类 号：R446.5[医药卫生—诊断学]