2016年6月至2017年4月辽宁省人感染H7N9禽流感病毒HA和NA基因特征分析  

Genetic characteristic of hemagglutinin and neuraminidase of human infected with H7N9 avian influenza virus,Liaoning,June 2016-April 2017

作　　者：石伟[1,2] 孙海波 王璐璐[2] 毛玲玲[2] 孙英伟[2] 姚文清[1,2] SHI Wei;SUN Hai-bo;WANG Lu-lu;MAO Ling-ling;SUN Ying-wei;YAO Wen-qing(Institute for Preventive Medicine,China Medical University,Shenyang,Liaoning 110005,China;不详)

机构地区：[1]中国医科大学预防医学研究院,辽宁沈阳110005 [2]辽宁省疾病预防控制中心感染与传染病预防控制所

出　　处：《现代预防医学》2020年第23期4348-4352,共5页Modern Preventive Medicine

基　　金：辽宁省自然科学基金计划重点项目(20170540488)。

摘　　要：目的本文对2016-2017年辽宁省人感染H7N9禽流感病毒血凝素(HA)和神经氨酸酶(NA)基因遗传进化特征分析。方法使用逆转录聚合酶链反应(RT-PCR)对选取的4株H7N9禽流感病毒株HA和NA基因进行扩增并测序,用生物信息学软件分析其基因进化变异特征。结果 2016年6月至2017年4月辽宁省4株毒株的HA和NA基因与WHO最新推荐的A/Hunan/02650/2016疫苗株的同源性最高,其HA基因的核苷酸和蛋白氨基酸序列同源性分别为99.3%~99.6%,99.8%~100%;NA基因的核苷酸和蛋白氨基酸序列同源性分别为97.7%~99.7%;98.3%~99.6%。本省所有毒株均属长江三角洲谱系,但主要聚集在两个次分支。4株病毒HA蛋白裂解位点333-342氨基酸序列均为PEIPKGR↓GLF,在338-339位点无连续的多个氨基酸插入(KRTA),属于低致病性禽流感病毒分子特征。HA受体结合位点均出现S138A、G186V、Q226L、T221P氨基酸的突变,NA茎部区均出现"QISNT"缺失,且NA蛋白上相关耐药位点上的氨基酸并未发生突变,3株毒株NA蛋白上糖基化位点第42位发生氨基酸序列NCSH→NCTH突变。结论 2016-2017年辽宁省人感染H7N9禽流感病毒HA和NA基因关键位点氨基酸发生突变,使病毒具有双受体结合特性以及毒力增强。Objective To analyze the genetic evolution characteristics of human infected with H7 N9 avian influenza virus hemagglutinin(HA)and neuraminidase(NA)in Liaoning province during 2016 to 2017.Methods The reverse transcriptase polymerase chain reaction(RT-PCR)was performed to amplify and sequence the HA and NA genes of the isolated four H7 N9 avian influenza virus strains.This genetic evolutionary and variation of virus were analyzed by using bioinformatics software.Results The HA and NA genes of 4 strains in Liaoning province during June 2016 to April 2017 had the highest homology with the latest recommended WHO/A/Hunan/02650/2016 vaccine strain,and the nucleotide and the amino acid sequence homology of HA gene were 99.3%-99.6%,99.8%-100%,respectively.The nucleotide and the amino acid sequence homology of NA gene were 97.7%-99.7%,98.3%-99.6%,respectively.All the virus strains in Liaoning province belonged to the Yangtze River Delta lineage,but they mainly cluster in two sub-branches.The amino acid sequences of the HA protein cleavage sites of the four virus strains were PEIPKGR↓GLF(333-342),and there were no consecutive multiple amino acid insertions(KRTA)at positions 338-339,which were molecular characteristics of low pathogenic avian influenza virus.Mutation S138 A,G186 V,Q226 L,T221 P at receptor binding sites were found in the HA,The NA protein stem region had"QISNT"deletions,and the amino acid at the relevant drug resistance site on the NA protein was not mutated,and the N-glycosylation sites mutations NCSH42 NCTH were found in the NA of 3 of 4 strains.Conclusion Human infected with H7 N9 avian influenza virus in Liaoning province were mutated at mutations in key sites of the HA and NA genes during 2016-2017,making the virus dual-receptor binding properties and enhanced virulence.

关 键 词：H7N9禽流感病毒 血凝素 神经氨酸酶 基因特征 

分 类 号：R511.7[医药卫生—内科学] R181.8[医药卫生—临床医学]