聚乙二醇模拟干旱胁迫下半夏茎段转录组分析  

Transcriptome analysis of stems of Pinellia ternata under drought stress simulated by PEG

作　　者：李铂[1,2] 彭亮 王楠[1,2] 孙晓春[1,2] 何懿菡 宋忠兴[1,2] 张永强 唐志书 黄文静[1,2] LI Bo;PENG Liang;WANG Nan;SUN Xiao-chun;HE Yi-han;SONG Zhong-xing;ZHANG Yong-qiang;TANG Zhi-shu;HUANG Wen-jing(Co-construction Collaborative Innovation Center of Chinese Medicine Resources Industrialization by Shaanxi&Education Ministry,Shaanxi University of Chinese Medicine,Xianyang 712083,China;State Key Laboratory of Research&Development of Characteristic Qin Medicine Resources(Cultivation),Xianyang 712083,China;College of Pharmacy,Shaanxi University of Chinese Medicine,Xianyang 712046,China)

机构地区：[1]陕西中医药大学陕西中药资源产业化省部共建协同创新中心,陕西咸阳712083 [2]秦药特色资源研究与开发国家重点实验室(培育),陕西咸阳712083 [3]陕西中医药大学药学院,陕西咸阳712046

出　　处：《中草药》2020年第21期5579-5589,共11页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81703652);现代农业产业技术体系建设专项资金项目(CARS-21);陕西省教育厅自然科学专项项目(18JK0223);咸阳市重大科技专项计划项目(2017k01-26)。

摘　　要：目的通过分析30%聚乙二醇(PEG)模拟的干旱胁迫处理下半夏茎段转录组数据,挖掘响应干旱胁迫的相关基因,探索干旱胁迫下半夏发生“倒苗”的分子机制。方法以干旱胁迫下半夏发生“倒苗”时的茎段作为研究材料,采用Illumina Hiseq 2500平台进行转录组双向测序,并对数据进行生物信息学相关分析。结果共获得23.00 Gb clean data,对照组与干旱胁迫处理组分别获得37467952和39903362个clean reads,经Trinity软件组装获得100274个uingenes。通过将组装所得unigenes分别与Nr、eggNOG、Pfam、Swiss-Prot、KOG、GO、KEGG、COG等数据库进行比对分析,41132个unigenes获得功能注释。最终筛选得到差异表达基因4052个,其中2080个DEGs表达上调,1972个DEGs表达下调。KEGG通路富集分析显示,差异表达基因主要集中在核糖体、碳代谢、淀粉和蔗糖代谢、植物激素信号转导和氨基酸生物合成等过程;基因表达水平分析显示,半夏中编码植物激素代谢与信号转导、氧化还原相关酶、热激蛋白和液泡加工酶等基因在干旱胁迫处理后表达水平显著上升,多数编码水通道蛋白的DEGs表达水平下降。结论通过对PEG模拟的干旱胁迫处理下半夏转录组测序结果进行分析,获得半夏水分代谢、激素代谢与信号转导、相关响应蛋白等候选基因,可为揭示干旱胁迫导致半夏“倒苗”的发生机制提供基因资源和理论依据。Objective In this study,the transcriptome profile of the stems from Pinellia ternata treated by 30%polyethylene glycol simulated drought stress was analyzed,the key enzyme genes responded to the drought stress were explored,so as to explore the molecular mechanism of P.ternata sprout tumble responded to the drought stress.Methods The stem segments of P.ternata under drought stress were used as research materials.The Illumina Hiseq 2500 platform was applied for transcriptome sequencing,and bioinformatics analysis was performed on the transcriptome data.Results A total of 23.00 Gb clean data was obtained,37467952 and 39903362 clean reads were gained from the control groups and the drought stress treatment,respectively,and 100274 uingenes were assembled by Trinity software.A total of 41132 unigenes were finally obtained with functional annotations against the Nr,eggNOG,Pfam,Swiss-Prot,KOG,GO,KEGG,and COG databases.Furthermore,4052 differentially expressed genes(DEGs)were obtained,in details,2080 DEGs were up-regulated while 1972 DEGs were with down-regulated expression.KEGG Pathway enrichment analysis showed that DEGs were mostly involved in ribosome,carbon metabolism,starch and sucrose metabolism,plant hormone signal transduction and biosynthesis of amino acids process.The expression levels of the genes encoded plant hormone metabolism and signal transduction,redox-related enzymes,heat shock proteins and vacuolar processing enzymes were significantly promoted after drought stress treatment,while most of the DEGs encoded aquaporins were down-regulated.Conclusion By analyzing the transcriptome profiles of P.ternata under drought stress simulated by PEG,the candidate genes associated with water metabolism,plant hormone and signal transduction,as well as the related responsive proteins were obtained,which could provide abundant genetic resources and theoretical basis for understanding the mechanism of P.ternata sprout tumble caused by drought stress.

关 键 词：半夏 聚乙二醇 干旱胁迫 转录组分析 差异表达基因 水分代谢 激素代谢 信号转导 

分 类 号：R282.12[医药卫生—中药学]