2种复壮方法对球孢白僵菌菌种复壮的影响分析  

Rejuvenation Results of Beauveria bassiana by Two Methods

作　　者：贾丽萍 王宏勋 陆俊宇 杨云波 高源 王兴 JIA Liping;WANG Hongxun;LU Junyu;YANG Yunbo;GAO Yuan;WANG Xing(Yuxi Bureau of Forestry and Grassland, Yuxi, Yunnan 653100,China;Yuxi Management Bureau of Hongtashan Nature Reserve, Yuxi, Yunnan 653100,China)

机构地区：[1]玉溪市林业和草原局,云南玉溪653100 [2]玉溪市红塔山自然保护区管护局,云南玉溪653100

出　　处：《林业调查规划》2020年第6期42-45,共4页Forest Inventory and Planning

摘　　要：采用活虫复壮法、虫尸复壮法对球孢白僵菌菌种进行复壮对比试验,并将2种方法复壮后的球孢白僵菌菌种接种在不同的培养基上,对比其长势与污染情况。结果表明:虫尸复壮法操作简单,污染小,转管容易,而活虫复壮法在害虫感病的过程中容易感染其他杂菌,需经过2~3次转管提纯才能使用;虫尸培养基是天然培养基,氮源更丰富,其菌株长势与污染情况均优于固体培养基。The rejuvenation test of Beauveria bassiana strains was carried out by living insect rejuvenation method and insect corpse rejuvenation method,and the growth and pollution of two rejuvenated Beauveria bassiana strains inoculated on different media were compared.The results showed that the insect corpse rejuvenation method was easy to operate and less pollution,while the living insect rejuvenation method was easy to infect other miscellaneous bacteria in the process of insect infection,which needed 2~3 times of tube transformation purification;the growth and pollution of the insect corpse culture medium which was natural medium,with more abundant nitrogen source,were better than solid medium.

关 键 词：球孢白僵菌 虫尸复壮法 活虫复壮法 菌株长势 培养基 

分 类 号：S763.1[农业科学—森林保护学] S718.8[农业科学—林学]