蛋白质结构解析技术研究现状与方向  

Research Direction and Current Situation of Protein Structure Analysis Technology

作　　者：徐颢溪 刘磊[2] Xu Hao-xi;Liu Lei(University of Science and Technology of China,Hefei Anhui 230027,China;Anhui Voeational and Technical Collge,Hefei Anhui 230011,China)

机构地区：[1]中国科学技术大学,安徽合肥230026 [2]安徽职业技术学院,安徽合肥230011

出　　处：《铜陵学院学报》2020年第5期106-109,共4页Journal of Tongling University

基　　金：安徽高等学校省级自然研究项目“巢湖流域中蓝藻碳代谢转录调控关键蛋白结构与功能的研究”(KJ2019A0988);安徹省高校学科(专业)拔尖人才学术资助项目“基于噬藻体对蓝藻特异性宿主细胞的感染机制”(gxyq2019178);安徽省高校优秀青年骨干人才国内外访学研修项目(gxfx2017213)。

摘　　要：蛋白质是生物体维持生命活动不可或缺的功能性大分子物质,解析蛋白质结构对于人们了解蛋白质的生物学功能及其生理特性具有重要意义。近些年来,蛋白质结构解析技术不断发展,除了传统意义上的X射线晶体衍射法和核磁共振技术等,具备高分辨率的冷冻电镜技术的出现令此领域有了革命性的进步。文章分析及比对了蛋白质结构解析技术的作用机制、技术进展以及它们未来的研究方向。Proteins are functional macromolecular substances indispensable for organisms to maintain life activities.The analysis of protein structure is of great significance for people to understand the biological functions and phyiological properties of proteins.'The protein strueture analysis technology has developed constantly in recent years.In addition to the traditional X--ray crystal diffraction method and the nuclear magnetie resonance technology,the emergence of the high-resolution cryo-electron microscopy technology has made revolutionary progress in this field.The paper analyzed and compared the mechanisms and technical developments of protein structure analysis technologies as well as their future research directions.

关 键 词：蛋白质结构 X射线晶体衍射 核磁共振 冷冻电镜 

分 类 号：Q51[生物学—生物化学]