基于NF-κB信号通路的细柱五加中impressic acid的抗炎作用研究  

Anti-inflammatory effect of impressic acid from Acanthopanax gracilistylus based on NF-κB signaling pathway

作　　者：肖瑾 李小军 黄建军[3] 高圣权 陆昌洙[5] 刘向前[1] XIAO Jin;LI Xiao-jun;HUANG Jian-jun;Ko Sung-kwon;Yook Chang-soo;LIU Xiang-qian(School of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China;College of Pharmaceutical Sciences,Gannan Medical University,Ganzhou 341000,China;Department of Clinical Biochemistry,Xiangya Medical College,Central South University,Changsha 410013,China;Department of Oriental Medical Food&Nutrition,Semyung University,Jecheon 27136,Korea;College of Pharmacy,Kyung Hee University,Seoul 130701,Korea)

机构地区：[1]湖南中医药大学药学院,湖南长沙410208 [2]赣南医学院药学院,江西赣州341000 [3]中南大学湘雅医学院,湖南长沙41001 [4]世明大学韩方食品营养学部,韩国堤川27136 [5]庆熙大学药学院,韩国首尔13070

出　　处：《中草药》2020年第22期5781-5787,共7页Chinese Traditional and Herbal Drugs

基　　金：湖南省自然科学基金资助项目(2019JJ40223,2019JJ50448);湖南中医药大学生物工程重点学科资助项目(校行科字[2018]3号);湖南省教育厅一般项目(18C0399)。

摘　　要：目的研究细柱五加Acanthopanax gracilistylus W.W.Smith中impressic acid(IA)对脂多糖(LPS)诱导的小鼠单核巨噬细胞RAW264.7的抗炎作用。方法以LPS诱导RAW264.7细胞建立炎症模型;使用EZ4U细胞增殖与细胞毒性分析试剂盒检测IA对RAW264.7细胞的毒性作用;Griess法测定一氧化氮(NO)水平;ELISA法测肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)水平;RT-PCR法检测TNF-α、IL-1βmRNA表达;Western blotting检测高迁移率族蛋白B1(HMGB1)蛋白表达;ELISA法细胞质和细胞核的核因子-κB(NF-κB)水平。结果Impressic acid可以显著抑制LPS诱导的RAW264.7细胞中TNF-α、IL-1β水平和HMGB1蛋白表达,并抑制NF-κB从细胞质向细胞核的转移(P<0.05、0.01)。结论细柱五加中IA对LPS诱导的RAW264.7细胞具有抗炎作用。Objective To explore the anti-inflammatory effect of impressic acid(IA)from Acanthopanax gracilistylus on lipopolysaccharide(LPS)-induced RAW264.7 cells.Methods RAW264.7 cells were stimulated by LPS to establish an inflammatory model.The cytotoxicity of IA on RAW 264.7 cells was detected by EZ4U cell proliferation and cytotoxicity analysis kit.The level of nitric oxide(NO)was determined by Griess.The levels of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)were determined by ELISA.The expressions of TNF-αand IL-1βmRNA were detected by RT-PCR.The expression of high-mobility group box 1 protein(HMGB1)was detected by western blotting.The levels of nuclear factor-κB(NF-κB)in cytoplasm and nucleus were measured by ELISA.Results IA significantly suppressed the levels of TNF-αand IL-1β,the expression of HMGB1 protein,and the translocation of NF-κB from cytoplasm to nucleus in LPS-induced RAW264.7 cells(P<0.05,0.01).Conclusion IA from A.gracilistylus has an anti-inflammatory effect on LPS-induced RAW264.7 cells.

关 键 词：细柱五加 impressic acid 三萜 抗炎 高迁移率族蛋白B1 核因子-κB 

分 类 号：R285.5[医药卫生—中药学]