尿TGF-β1、8-OHdG检测在早期糖尿病肾病中的应用价值  

Application value of urine TGF-β1 and 8-OHdG detection in early diabetic nephropathy

作　　者：冒艳阳 杨侠宇 魏杰 冯健 郭正勇 MAO Yanyang;YANG Xiayu;WEI Jie;FENG Jian;GUO Zhengyong(Department of Clinical Laboratory,Nanjing Meishan Hospital,Nanjing,Jiangsu 210039,China;Department of Nephrology,Nanjing Meishan Hospital,Nanjing,Jiangsu 210039,China)

机构地区：[1]南京梅山医院检验科,江苏南京210039 [2]南京梅山医院肾内科,江苏南京210039

出　　处：《检验医学与临床》2020年第24期3565-3567,3573,共4页Laboratory Medicine and Clinic

基　　金：江苏省南京市卫生科技发展专项资金项目(YKK18224)。

摘　　要：目的探讨尿转化生长因子-β1(TGF-β1)及8-羟基脱氧鸟嘌呤(8-OHdG)检测在早期糖尿病肾病(DN)中的应用价值。方法选取2019年1—11月该院收治的2型糖尿病患者120例作为研究对象,根据尿微量清蛋白与肌酐比值(ACR)将其分为3组,分别为正常蛋白尿组(ACR<30 mg/g)、微量蛋白尿组(30 mg/g≤ACR<300 mg/g)、大量蛋白尿组(ACR≥300 mg/g)。另选取同期30例健康体检者作为对照组。比较各组TGF-β1、8-OHdG、ACR、血肌酐水平及TGF-β1、8-OHdG单独与联合检测的阳性率。结果与对照组比较,微量蛋白尿组、大量蛋白尿组ACR水平明显升高,差异有统计学意义(P<0.05)。正常蛋白尿组、微量蛋白尿组、大量蛋白尿组TGF-β1、8-OHdG水平高于对照组,且随尿蛋白水平的增加TGF-β1、8-OHdG水平呈递增趋势,各组间比较,差异均有统计学意义(P<0.05)。各组中TGF-β1、8-OHdG联合检测的阳性率均高于TGF-β1、8-OHdG单独检测,差异有统计学意义(P<0.05)。结论尿TGF-β1、8-OHdG检测能够较灵敏地反映DN患者早期肾损害,两者联合检测在DN早期筛查中具有一定价值,值得临床推广应用。Objective To explore the application value of urine transforming growth factor-β1(TGF-β1)and 8-hydroxydeoxyguanine(8-OHdG)in early diabetic nephropathy(DN).Methods A total of 120 patients with type 2 diabetes admitted to the hospital from January to November 2019 were selected as the research objects.According to the ratio of microalbuminuria to creatinine(ACR),they were divided into 3 groups:normal albuminuria group(ACR<30 mg/g),microalbuminuria group(30 mg/g≤ACR<300 mg/g)and large albuminuria group(ACR≥300 mg/g).Another 30 healthy people underwent physical examination in the same period were selected as the control group.The TGF-β1,8-OHdG,ACR,serum creatinine levels and the positive rates of TGF-β1 and 8-OHdG detected alone or in combination were compared.Results Compared with the control group,the ACR level in microalbuminuria group and large albuminuria group was significantly increased,and the difference was statistically significant(P<0.05).The levels of TGF-β1 and 8-OHdG in normal albuminuria group,microalbuminuria group and large albuminuria group were higher than those in control group,and the levels of TGF-β1 and 8-OHdG showed an increasing trend with the increase of albuminuria level,and the differences were statistically significant(P<0.05).The positive rate of combined detection of TGF-β1 and 8-OHdG in each group was higher than that of TGF-β1 and 8-OHdG alone detection,and the differences were statistically significant(P<0.05).Conclusion Urine TGF-β1 and 8-OHdG detection can sensitively reflect the early renal damage in patients with DN.The combined detection of the two indexes has a certain value in the early screening of DN and is worthy of clinical application.

关 键 词：糖尿病肾病 转化生长因子-Β1 8-羟基脱氧鸟嘌呤 尿微量清蛋白与肌酐比值 

分 类 号：R446.6[医药卫生—诊断学]