连夏宁心方对心肌梗死大鼠左室重构及JAK1/STAT3信号通路的影响  

Effects of Lianxia Ningxin Prescription on Left Ventricular Remodeling and JAK1/STAT3 Signal Pathway in Rats with Myocardial Infarction

作　　者：李赛赛 杨阳 李家立[2] 戴方圆 李芳赫 李平 LI Saisai;YANG Yang;LI Jiali;DAI Fangyuan;LI Fanghe;LI Ping(Third Affiliated Hospital of Beijing University of Chinese Medicine,Beijing 100029,China;Affiliated Hospital of Jining Medical University,Jining 272029,China)

机构地区：[1]北京中医药大学第三附属医院,北京100029 [2]济宁医学院附属医院,山东济宁272029

出　　处：《中国中医药信息杂志》2020年第12期48-52,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81960852);山东省自然科学基金(ZR2017BH063)。

摘　　要：目的观察连夏宁心方对心肌梗死大鼠左室重构及JAK1/STAT3信号通路的影响,探讨其相关作用机制。方法采用结扎左冠状动脉前降支建立大鼠心肌梗死模型,将造模成功的18只SD大鼠随机分为模型组、连夏宁心方组、塞来昔布组,另设假手术组,每组6只。连续给药28 d,超声心动图测量大鼠心脏结构和功能,Masson染色检测心肌胶原容积分数(CVF),HE染色观察心肌细胞形态学变化,Western blot检测JAK1、STAT3、核因子-κB(NF-κB)蛋白的表达。结果与假手术组比较,模型组大鼠左室射血分数(EF)和缩短分数(FS)显著下降,左室舒张末期内径(LVIDd)和左室收缩末期内径(LVIDs)显著增加,心肌纤维增生明显,胶原过度沉积,心肌细胞排列紊乱,心肌间质大量炎性细胞浸润,JAK1、STAT3、NF-κB蛋白表达显著升高(P<0.01);与模型组比较,连夏宁心方组EF和FS升高,LVIDd降低,CVF明显下降,心肌细胞排列较为整齐,炎性细胞浸润程度明显减轻,JAK1、STAT3、NF-κB蛋白表达显著降低(P<0.05,P<0.01)。结论连夏宁心方可改善心肌梗死大鼠左室重构,其机制可能与下调JAK1/STAT3信号通路的表达有关。Objective To observe the effects of Lianxia Ningxin Prescription on left ventricular remodeling and JAK1/STAT3 signal pathway in rats with myocardial infarction;To explore its related mechanism of action.Methods The model of myocardial infarction was established by ligating the anterior descending coronary artery.Totally 18 SD rats with successful modeling were randomly divided into model group,Lianxia Ningxin Prescription group,celecoxib group,and another 6 rats in sham-operation group,with 6 rats in each group.After 28 days of continuous treatment,echocardiography was used to measure cardiac structure and function in rats.Masson staining was used to detect myocardial collagen volume fraction(CVF).HE staining was used to observe the morphological changes of cardiomyocytes.Western blot was used to detect the expressions of JAK1,STAT3 and NF-κB proteins.Results Compared with the sham-operation group,the left ventricular ejection fraction(EF)and fractional shortening(FS)of the model group significantly decreased,and the left ventricular end-diastolic inner diameter(LVIDd)and left ventricular end-systolic inner diameter(LVIDs)significantly increased,myocardial fiber hyperplasia,excessive collagen deposition,myocardial cell arrangement disorder,and myocardial interstitial inflammatory cell infiltration appeared.Expressions of JAK1,STAT3,NF-κB protein significantly increased(P<0.01).Compared with the model group,the EF and FS significantly increased in Lianxia Ningxin Prescription group,while LVIDd and CVF were reduced.Myocardial cells were arranged neatly.The degree of inflammatory cell infiltration was significantly reduced,and expressions of JAK1,STAT3,and NF-κB protein were significantly reduced(P<0.05,P<0.01).Conclusion Lianxia Ningxin Prescription can improve left ventricular remodeling in rats with myocardial infarction,and its mechanism may be related to the down-regulation of expression of JAK1/STAT3 signal pathway.

关 键 词：连夏宁心方 心肌梗死 左室重构 JAK1/STAT3信号通路 大鼠 

分 类 号：R285.5[医药卫生—中药学]