survivin shRNA-APC双基因对人HT-29结肠癌细胞中c-myc、hTERT表达的影响  

Effects of Survivin shRNA-APC on c-myc and hTERT Expression in Human HT-29 Colon Cancer Cells

作　　者：袁喜先 陈月[2] Yuan Xixian;Chen Yue(The First Affiliated Hospital of Jiamusi University,Jiamusi 154003;Jiamusi University,Jiamusi 154007,China)

机构地区：[1]佳木斯大学附属第一医院消化科,黑龙江佳木斯154003 [2]佳木斯大学,黑龙江佳木斯154007

出　　处：《广东化工》2020年第22期53-54,40,共3页Guangdong Chemical Industry

摘　　要：本课题探讨Survivin shRNA-APC双基因对HT-29结肠癌细胞裸鼠皮下转移瘤组织中c-myc、hTERT(人端粒酶逆转录酶)表达的影响。方法选取30只裸鼠随机抽分为5组,分别为Survivin shRNA-APC双基因组、Survivin shRNA单基因组、APC单基因组、空载组和空白组。依次将本实验组前期构建好的各组稳转株及人HT-29结肠肿瘤细胞注射到各组实验动物的左前腋下,等待成瘤。成瘤后,剥离组织,进行Real-time PCR及免疫组化,检测结肠肿瘤细胞中c-myc、hTERT mRNA及蛋白的表达结果。结果将c-myc、hTERT(人端粒酶逆转录酶)的mRNA及蛋白结果进行统计分析,在SurvivinshRNA-APC双基因组、SurvivinshRNA单基因组、APC单基因组中含量均下降。双基因组与单基因组相比较,c-myc、hTERT的mRNA及蛋白表达含量变化更为明显。空载组、空白组相比较差异无统计学意义(P>0.05)结论SurvivinshRNA-APC双基因能够下调c-myc、hTERT的表达发挥抑制肿瘤细胞增殖的作用,并且能说明双基因对结肠癌的抑制效果优于SurvivinshRNA单基因组和APC单基因组。Objective to study the Survivin shRNA APC were steady turn strains to C-MYC and the influence of the expression of hTERT(human telomerase reverse transcriptase)in nude mice with HT-29 colon cancer cell subcutaneous metastasis.Methods 30 rats were randomly selected into 5 groups,including Survivin shRNA-APC double genome,Survivin shRNA single genome,APC single genome,no-load group and blank group.The Survivin shRNA-APC,Survivin shRNA-APC,APC,Will light colon cancer cells and HT-29 colon cancer cells injected into the left anterior axillary groups of nude mice,respectively,to await tumor formation.After tumor formation,the tissues were exfoliated,real-time PCR and immunohistochemistry were performed,determination of C-MYC in transplanted tumor tissue cells and hTERT mRNA and protein expression.Results mRNA and protein expression of Both C-MYC and hTERT were correlated with the expression levels of survivin-shRNA-APC.,the Survivin-shRNA single genome,and the APC single genome were all reduced when compared with the no-load and no-load groups.Compared with single genome,mRNA and protein expression levels of C-MYC and hTERT were more obvious.There was no obvious difference between no-load group and blank group(P>0.05).Conclusion The survivinl-shRNA-APC genes would down-regulate the expression of C-MYC and hTERT and inhibit the proliferation of tumor cells,and the results indicated that the inhibition effect of the survivin-shRNA and APC genes on colon cancer was superior to that of the survivin-shRNA and APC genomes.

关 键 词：结肠癌 C-MYC hTERT(人端粒酶逆转录酶) 双基因 细胞增殖 

分 类 号：TQ[化学工程]