缺氧神经细胞中“SNHG15-miR-24-神经蛋白聚糖”调控轴的作用  

The Role of“SNHG15-Mir-24-neurocan”in Regulating the Axis in Hypoxic Nerve Cells

作　　者：逯琦 冀方超 李晓楠 王英 孙兴元 Lu Qi;Ji Fangchao;Li Xiaonan;Wang Ying;Sun Xingyuan(Jiamusi University Clinical Medical College,Jiamusi 154002;The Third Affiliated Hospital of Qiqihar Medical College,Qiqihar 161000,China)

机构地区：[1]佳木斯大学临床医学院,黑龙江佳木斯154002 [2]齐齐哈尔医学院附属第三医院,黑龙江齐齐哈尔161000

出　　处：《广东化工》2020年第22期236-237,共2页Guangdong Chemical Industry

摘　　要：目的:研究缺氧神经细胞中“SNHG15-miR-24-神经蛋白聚糖(Neurocan)”调控轴中的作用及相关性。方法:低氧培养人神经母细胞瘤细胞SH-SY5Y,分别在干预24 h、48 h、72 h收集细胞,通过CCK8法检测SNHG15对SH-SY5Y细胞增殖的影响,qPCR检测LncRNA SNHG15及miRNA-24表达,WB检测Neurocan的蛋白水平。结果:低氧培养时可促进SNHG15表达上调(t=-22.106,P=0.001),并下调miRNA-24表达(t=10.922,P=0.008),过表达miR-24能够下调Neurocan的表达,能够促进低氧培养下SH-SY5Y的增殖(P<0.05)。结论:“SNHG15-miR-24-神经蛋白聚糖”调控轴可用于急性脑梗塞的早期预警及病情评估,为临床提供参考依据。Objective To investigate the role and correlation of“SNHG15-miR-24-neurocan”in the regulatory axis of hypoxic nerve cells.Methods Human neuroblastoma sh-SY5Y cells were cultured with hypoxia,and the cells were collected at 24 h,48 h and 72 h after intervention,respectively.The effect of SNHG15 on sh-SY5Y cell proliferation was detected by CCK8 method,the expressions of LncRNA SNHG15 and miRNA-24 were detected by qPCR,and the protein level of Neurocan was detected by WB.Results During hypoxic culture,the expression of SNHG15 was up-regulated(T=-22.106,P=0.001)and the expression of miRNA-24 was down-regulated(T=10.922,P=0.008).Overexpression of miRNA-24 could down-regulate the expression of Neurocan and promote the proliferation of SH-SY5Y under hypoxic culture(P<0.05),suggesting that down-regulation of Neurocan could reduce the damage of hypoxic culture on nerve cells.Conclusion“SNHG15-miR-24-neurocan”regulatory axis can be used for early warning and condition assessment of acute cerebral infarction,providing clinical reference.

关 键 词：急性脑梗死 LncRNA SNHG15 miRNA-24 神经蛋白聚糖 

分 类 号：TQ[化学工程]