‘艳丽’草莓微繁殖苗与普通苗的表型差异及转录组分析  

Phenotypic variation between micro-propagated and conventional plants of‘Yanli’strawberry and its mechanism revealed by transcriptome analysis

作　　者：朱天姝 王保田 张志宏[1] ZHU Tianshu;WANG Baotian;ZHANG Zhihong(College of Horticulture,Shenyang Agricultural University/Liaoning Key Laboratory of Strawberry Breeding and Cultivation,Shenyang 110866,Liaoning,China)

机构地区：[1]沈阳农业大学园艺学院·辽宁省草莓育种与优质栽培重点实验室,沈阳110866

出　　处：《果树学报》2020年第12期1836-1845,共10页Journal of Fruit Science

基　　金：国家自然科学基金(31672113);国家重点研发计划课题(2018YFD1000102);“兴辽英才计划”项目(XLYC1902069)。

摘　　要：【目的】分析草莓微繁殖苗与普通苗表型差异,通过转录组测序揭示草莓微繁殖苗与普通苗差异表达的基因,探索草莓微繁殖苗与普通苗表型差异的调控机制。【方法】以草莓品种‘艳丽’为试材,在繁苗期和栽培期调查微繁殖苗与普通苗之间的表型差异,并以茎尖生长点为试材进行转录组测序分析。【结果】在繁苗期,微繁殖原种苗的匍匐茎数、新茎数、叶片数均明显高于普通苗,而株高、冠径、叶面积差异不明显。在栽培期,微繁殖一代苗比普通苗开花结果略早,但是两者在果实大小、产量、可溶性固形物含量上没有明显差异。微繁殖原种苗与普通苗的茎尖生长点里有1803个基因显著差异表达,相对于普通苗,微繁殖原种苗中上调表达基因1469个,下调表达基因334个。差异表达的基因涉及光合作用、植物激素合成及信号转导、植物病原菌互作等。【结论】‘艳丽’草莓微繁殖苗的繁苗能力明显高于普通苗,这一表型差异与植物激素合成及信号转导通路上基因的差异表达有关。【Objective】Strawberry,the perennial herb of the family Rosaceae,is widely cultivated in the world due to high economic and nutritional value.Currently,the area of cultivation of strawberry is increasing rapidly in China.Strawberry plants are mainly propagated by runners which would lead to spreading of some diseases.Therefore,micro-propagation technology has been used to generate diseasefree plants to improve the quality of products.Some studies have reported that micro-propagated strawberry plants have more runners,leaves,and even high net photosynthetic rate.However,the molecular mechanism of the differences is rarely reported.In this study,we investigated the differentially expressed genes between the micro-propagated and the conventional plants of strawberry cultivar‘Yanli’through transcriptomics analysis.【Methods】The micro-propagated stock plants of cultivated strawberry(Fragaria×ananassa Duch.)cultivar‘Yanli’were transplanted and were compared with the conventional plants propagated by runner mode in the field.Some of the investigations and analysis were as follows:(1)Investigation of morphological indexes during the vegetative propagation stage.The micropropagated stock plants and the conventional plants were planted in the pots in open field,and the number of leaves,branch crowns,runners,etc.were recorded every 14 days.(2)Phenological observation.The first generation of the micro-propagated stock plants and the conventional plants were cultivated in solar greenhouse,and the growth and development status were investigated every 3 days and the first flowering date,flower blooming date,and the fruit ripening date were recorded.(3)Investigation of morphological indexes during the flowering and fruiting stage.Plant height,plant diameter,number of leaves and leaf area were measured from 30 plants in the period of the beginning of the flowering,fruit ripening,and the end of the first fruit harvest.(4)Fruit yield and quality.The hardness and soluble solid content of the first fruit were measured

关 键 词：草莓 微繁殖 转录组测序 

分 类 号：S668.4[农业科学—果树学]