决明子醇提物对胰岛β细胞凋亡的影响及其机制  

The effect of Ethanol extraction from Semen Cassiae on the apoptosis of pancreatic beta cells and its mechanism

作　　者：白兰 李思奇 张翼麒 张雪妍 肖镜怡 张杰[1] BAI Lan(Mudanjiang Medical College,Mudanjiang 157011,China)

机构地区：[1]牡丹江医学院,黑龙江牡丹江157011

出　　处：《牡丹江医学院学报》2020年第6期1-3,共3页Journal of Mudanjiang Medical University

基　　金：黑龙江省牡丹江医学院学生科研项目(201902)。

摘　　要：目的研究决明子醇提物(Ethanol extraction from Semen Cassiae,ESC)对高脂培养的胰岛β细胞的保护作用,并探讨其作用机制。方法将胰岛β细胞随机分为4组:对照组(RPMI-1640完全培养基)、模型组(0.75 mmol/L棕榈酸)、低剂量ESC组(15 mg/L ESC+0.75 mmol/L棕榈酸)、高剂量ESC组(30 mg/L ESC+0.75 mmol/L棕榈酸),用相应培养基培养24 h。采用MTT法检测各组胰岛β细胞生长抑制率,应用荧光显微镜检测细胞凋亡率。采用RT-PCR检测bcl-2、caspase-3 mRNA的相对表达量。结果与模型组比较,低剂量ESC组和高剂量ESC组胰岛βTc3细胞抑制率和凋亡率明显降低(P<0.01)。与模型组比较,低剂量ESC组和高剂量ESC组胰岛βTc3细胞的bcl-2基因表达显著增加,caspase-3基因表达显著降低(P<0.01)。结论ESC可通过调控bcl-2和caspase-3基因表达,从而抑制高脂诱导的βTc3细胞凋亡。Objective To study the protective effect and mechanism of Ethanol extraction from Semen Cassiae(ESC)on pancreatic beta cells in saturated fatty acids.Methods PancreaticβTc3 cells were randomly divided into control group(RPMI-1640 medium),model group(0.75 mmol/L palmitic acid),low dose ESC group(15 mg/L ESC+0.75 mmol/L palmitic acid)and high dose ESC group(30 mg/L ESC+0.75 mmol/L palmitic acid).The cells inhibition rate was detected by MTT method and the apoptosis rate was detected by fluorescence microscope.The mRNA expression levels of bcl-2 and caspase-3 in all groups were detected by RT-PCR.Results Compared to model group,the apoptotic rate and proliferation inhibition rate ofβTc3 cells were significantly declined in low dose ESC group and high dose ESC group.Compared to model group,the level of bcl-2 gene expression was significantly increased,and caspase-3 was significantly decreased in low dose ESC group and high dose ESC group.Conclusion ESC could inhibit the apoptotic rate ofβTc3 cells probably through regulating bcl-2 and caspase-3 gene expression.

关 键 词：决明子 胰岛细胞 细胞凋亡 ΒTC3细胞 

分 类 号：R329.21[医药卫生—人体解剖和组织胚胎学]