Relationship between White-partridge Feather Trait and SLC45A2 Polymorphism in Wenchang Chicken  

作　　者：He Yiping Chen Jie Gao Xinfeng Xu Jiguo 

机构地区：[1]Guangzhou Changyuan Biological Feed Co.Ltd,.Guangzhou 510545,China [2]Institute of Biotechnology,Nanchang Normal University/Jiangxi Key Laboratory of Chicken Genetic Improvement/Chinese Academy of Sciences-Poultry Molecular Breeding Technology Joint Laboratory,Nanchang 330022,China [3]Guangzhou Kwang Feng Industrial Co.,Ltd.,Guangzhou 510450,China [4]College of A nimal Sciences,South China A gricultural University,Guangzhou 510642,China

出　　处：《Animal Husbandry and Feed Science》2020年第3期9-13,共5页动物与饲料科学（英文版）

基　　金：Supported by Science and Technology Project of Jiangxi Provincial Department of Education(GJJ181080);Start-up Fund of Nanchang Normal University(GJJ181080);"11531"Construction Project of Nanchang Normal University.

摘　　要：[Objective]The paper was to study the inheritance of white-partridge feather trait in Wenchang chicken and identify the related genetic variation,so as to provide references for the utilization of white-partridge population of Wenchang chicken and molecular marker-assisted selection of white-partridge feather trait.[Method]The inheritance of white-partridge feather trait was studied by reciprocal cross test.SLC45A2 was selected as a candidate gene based on the genetic characteristics of white-partridge feather and results of previous researches.The variation in the exon domain of SLC45A2 gene was analyzed by re-sequencing method.The relationship between missense mutation/nonsense mutation in the exon domain and white-partridge feather trait was verified.[Result]Reciprocal cross test showed that white-partridge feather trait was associated recessive inheritance compared with yellow-partridge feather.The amplification results indicated that there were five SNPs in SLC45A2 gene,in which three were located in exon domain,including two missense mutations(c.a74g and c.gll54c)and one synonymous mutation(c.c561t).The missense mutation was verified with Wenchang chicken Bawang chicken,Qingyuan partridge chicken,Xinghua chicken and red jungle fowl as materials.The results demonstrated that c.g1154c locus of SLC45A2 gene was completely linked with white-partridge feather trait.[Conclusion]The trait of white-partridge feathers showed recessive inheritance and c.g1154c locus might be the causation.

关 键 词：Wenchang chicken White-partridge feather SLC45A2 gene Silver feather locus 

分 类 号：S831.2[农业科学—畜牧学]