抗根肿病甘蓝型油菜新品种华油杂62R的选育  

Breeding of a novel clubroot disease-resistant Brassica napus variety Huayouza 62R

作　　者：李倩 Nadil Shah 周元委 侯照科 龚建芳 刘珏 尚政伟 张磊 战宗祥 常海滨 傅廷栋[1] 朴钟云[5] 张椿雨[1] LI Qian;Nadil Shah;ZHOU Yuan-Wei;HOU Zhao-Ke;GONG Jian-Fang;LIU Jue;SHANG Zheng-Wei;ZHANG Lei;ZHAN Zong-Xiang;CHANG Hai-Bin;FU Ting-Dong;PIAO Zhong-Yun;ZHANG Chun-Yu(College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,Hubei,China;Yichang Academy of Agricultural Sciences,Yichang 443000,Hubei,China;Dehong Dai Jingpo Autonomous Prefecture Agricultural Technology Extension Center,Dehong 678400,Yunnan,China;Huanggang Academy of Agricultural Sciences,Huanggang 438000,Hubei,China;College of Horticulture,Shenyang Agricultural University,Shenyang 110000,Liaoning,China)

机构地区：[1]华中农业大学植物科学技术学院,湖北武汉430070 [2]宜昌市农业科学研究院,湖北宜昌443000 [3]德宏傣族景颇族自治州农业技术推广中心,云南德宏678400 [4]黄冈市农业科学研究院,湖北黄冈438000 [5]沈阳农业大学园艺学院,辽宁沈阳110000

出　　处：《作物学报》2021年第2期210-223,共14页Acta Agronomica Sinica

基　　金：国家重点研发计划项目(2016YFD0101300);国家现代农业产业技术体系建设专项(CARS-12)资助。

摘　　要：我国油菜根肿病发病面积在66.7万公顷左右,约占总生产面积的10%,已严重威胁到油菜的安全生产。基于此,本研究以含有CRb抗根肿病位点的大白菜材料CR Shinki为供体亲本,以甘蓝型油菜国审杂交种华油杂62的父本Pol.CMS恢复系Bing409为受体亲本,通过杂交、回交及自交等育种程序,结合前景和遗传背景筛选,将CRb抗病位点导入到Bing409中。在BC3F2代获得了遗传背景高度接近Bing409且含CRb抗病位点的抗根肿病新恢复系Bing409R,进而成功选育了我国首个抗根肿病杂交油菜新品种华油杂62R。CRb抗病位点在甘蓝型油菜背景中表现为单基因显性遗传,根肿病抗性遗传改良的同时并未对Bing409R及由其配制的杂交种华油杂62R的产量、品质造成不良影响,Bing409R及华油杂62R对我国四川、湖北、安徽等地区根肿菌生理小种具有免疫抗性。本研究的开展为我国油菜抗根肿病育种提供了宝贵资源,为我国抵抗油菜根肿病的威胁提供了重要保障。The rapeseed clubroot disease incidence in China is about 0.67 million hectare,accounting for 10%of the canola production area,which become a serious threat for the safety of Brassica napus industry.Based on this,we used CR Shinki,a Chinese cabbage material containing CRb clubroot disease resistance locus,as the donor parent,and Pol.CMS restorer line Bing409,the parent of Brassica napus national approved varieties Huayouza 62,as the recipient parent,and the CRb resistance locus was introduced into Bing409 by breeding programs such as crossing,backcrossing,self-cross with the foreground and genetic background selection.In the BC3F2 generation,a new restorer line Bing409R with a genetic background close to Bing409 containing CRb resistance locus was obtained,and Huayouza 62R,the first rapeseed hybrid resistant to clubroot disease in China was successfully developed.The results were as follows:CRb disease resistance locus appeared as a dominant single-gene inheritance in B.napus background,and the genetic improvement of resistance to clubroot disease did not at the expense of yield and quality losses for new restorer line Bing409R and its hybrid Huayouza 62R.Bing409R and Huayouza 62R were showed immune-resistance to physiological races of Plasmodiophora brassicae in Sichuan,Hubei,and Anhui provinces in China.This study will provide valuable resources for the breeding of rapeseed in China,and supplemented important support to overcome the threat of rapeseed clubroot disease.

关 键 词：甘蓝型油菜 根肿病 CRb抗病位点 恢复系 分子改良 华油杂62R 

分 类 号：S435.654[农业科学—农业昆虫与害虫防治]