山西省古交市大豆胞囊线虫新小种X12分布调查  

Distribution survey of soybean cyst nematode of new race X12 in Gujiao city,Shanxi province

作　　者：练云[1] 王金社[1] 魏荷[1] 李金英[1] 弓贵明 王树峰[1] 张晶鹏 李茂林 郭建秋 卢为国[1] LIAN Yun;WANG Jin-She;WEI He;LI Jin-Ying;GONG Gui-Ming;WANG Shu-Feng;ZHANG Jing-Peng;LI Mao-Lin;GUO Jian-Qiu;LU Wei-Quo(Institute of Industrial Crops,Henan Academy of Agricultural Sciences/Zhengzhou Subcenter of National Soybean Improvement Center/Key Laboratory of Oil Crops in Huanghuaihai Plain,Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crops Inprovement,Zhengzhou 450002,Henan,China;Gujiao Seed Service Station,Taiyuan 030200,Shanxi,China;Luoyang Academy of Agriculture and Forestry Sciences,Luoyang 471023,Henan,China)

机构地区：[1]河南省农业科学院经济作物研究所/国家大豆改良中心郑州分中心/农业农村部黄淮海油料作物重点实验室/河南省油料作物遗传改良重点实验室,河南郑州450002 [2]古交市种子服务站,山西太原030200 [3]洛阳农林科学院,河南洛阳471023

出　　处：《作物学报》2021年第2期237-244,共8页Acta Agronomica Sinica

基　　金：河南省科技攻关项目(202102110158);中原科技创新领军人才工程(194200510003);国家自然科学基金项目(31901501)资助。

摘　　要：X12是具有超强致病力的大豆胞囊线虫(SCN)新小种,于2012年在山西省古交市邢家社首次发现,该小种对大豆生产有巨大威胁。定期调查X12生理小种分布,对有目的地采取防治措施阻止X12小种扩散有重要意义。本研究于2019—2020年调查古交市土样,利用Riggs模式鉴定生理小种,绘制古交市X12生理小种分布图,探讨其周围生理小种类型及分布规律。结果表明,在采集的受SCN感染的33份样本中,26份鉴定出生理小种类型,占采集样本的78.8%;2号和4号生理小种在该地区分布广泛,2号小种检出频率为57.7%;4号小种检出频率为42.3%。4号小种群体能够侵染优异抗源兴县灰皮支(ZDD2315)且胞囊指数(female index,FI)大于10,即被认定为X12小种,在此次鉴定为4号小种的11份样本中,有2份进一步鉴定为X12小种,含重复采集2012年在邢家社发现的X12样本;另有3份鉴定为4号小种的样本,其SCN群体能在兴县灰皮支上寄生,但FI未达到10。这26份样本的SCN群体能在Peking和PI88788上寄生,且FI>50的分别占73.1%和57.7%。表明,除邢家社发现有X12小种,在河口镇也发现了X12小种;在邢家社周围810 km2仅检测到2号和4号小种;有3份样本的SCN群体有可能会优先由4号小种进化为X12小种。建议在古交市采取有力、有效措施减缓SCN的致病力升级及X12小种扩散。X12 was a new race of soybean cyst nematode(SCN)with super strong pathogenicity and it was first detected in Xingjiashe county,Gujiao city,Shanxi province,China in 2012,which was a huge threat to soybean production.The research of race X12 distribution is meaningful for developing management strategies to prevent race X12 population from spreading.Therefore,a survey for the distribution of soybean cyst nematode race X12 in Gujiao city was conducted in 2019 and 2020.A distribution of races was constructed based on Riggs model.The race distribution was discussed in this study.A total of 33 soil samples infected soybean cyst nematode were collected.Twenty-six were identified as physiological subtypes,accounting for 78.8%of the samples,in which race 2 and race 4 accounted for 57.7%(15 samples)and 42.3%(11 samples),respectively.In general,race X12 was determined if race 4 population virulent on ZDD2315 accession with female index(FI)>10.In 11 samples determined as race 4,including two samples were further determined as race X12 including the sample collected from Xingjiashe location,which was the original location detected as race X12 in 2012.There were three other SCN populations which determined as race 4 could virulent on ZDD2315 but with FI<10.There were 73.1%and 57.7%of populations with FI>50 among 26 evaluated populations on Peking and PI88788,respectively.The results showed that race X12 population were also detected in Hekouzhen except Xingjiashe.Only race 2 and race 4 were detected around Xingjiashe,covered 810 km^2.SCN populations with 3 samples were likely to preferentially evolve from subspecies 4 to subspecies X12.The results showed that strong and effective measures should be taken in Gujiao city to slow down the virulence escalation of SCN and the spread of X12 species.

关 键 词：大豆 大豆胞囊线虫 X12 调查 生理小种 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]