雌激素受体基因多态性与鲁南地区汉族妇女卵巢反应性的相关性研究  

Study on the Relationship between Estrogen Receptor Gene Polymorphism and Ovarian Response in Women of Han nationality in the South of Shandong Province

作　　者：邱欣[1] 王伟[1] 吴秋红 薛子成[1] 李雨[1] 刘卫东[1] QIU Xin;WANG Wei;WU Qiuhong(Department of Pharmacy,the Hospital of Zaozhuang Maternal and Child Health,Zaozhuang City,Shandong Province 277000)

机构地区：[1]山东省枣庄市妇幼保健院药学部,277000

出　　处：《医学理论与实践》2020年第24期4059-4061,4086,共4页The Journal of Medical Theory and Practice

基　　金：山东省医药卫生科技发展计划(2017WS385)。

摘　　要：目的:探讨雌激素受体(ER)基因多态性与鲁南地区汉族妇女卵巢反应性的相关性。方法:采用荧光染色原位杂交技术检测100例不孕症患者DNA的ERα基因的PvuⅡ(rs2234693)、XbaⅠ(rs9340799)和ERβ基因的RsaⅠ(rs1256049)和AluⅠ(rs4986938)单核苷酸多态点基因型,分析其基因型和等位基因频率在高反应组、低反应组和正常组间的分布差异,探讨ER基因多态性与卵巢反应性的相关性。结果:高反应组和低反应组患者ERα基因位点PvuⅡ(rs2234693)基因型频率与等位基因频率与正常组之间的差异无统计学意义(P>0.05)。高反应组和低反应组患者ERα基因位点XbaⅠ(rs9340799)基因型频率与正常组之间的差异无统计学意义(P>0.05)。高反应组患者ERα基因位点XbaⅠ(rs9340799)等位基因频率与正常组之间的差异无统计学意义(P>0.05)。而低反应组患者ERα基因位点XbaⅠ(rs9340799)等位基因频率与正常组之间的差异具有统计学意义(P<0.01)。高反应组和低反应组患者ERβ基因位点RsaⅠ(rs1256049)和AluⅠ(rs4986938)的基因型频率和等位基因频率与正常组之间的差异无统计学意义(P>0.05)。结论:ERα基因位点XbaⅠ(rs9340799)基因多态性与鲁南地区汉族妇女COH中的卵巢低反应相关。XbaⅠ(rs9340799)位点G等位基因可增加卵巢低反应的风险。Objective:To explore the relationship between estrogen receptor(ER)gene polymorphism and ovarian response in Han women in the south of Shandong province.Methods:The ERαgene PvuⅡ(rs2234693),XbaⅠ(rs9340799)and ERβgene RsaⅠ(rs1256049)and AluⅠ(rs4986938)single nucleotide polymorphism(SNP)genotypes of DNA were detected by fluorescence staining in situ hybridization.The distribution differences of genotype and allele frequency between high response group,low response group and normal group were analyzed,and the relationship between ER gene polymorphism and ovarian response was discussed.Results:There was no significant difference in the genotype frequency and allele frequency of ERαlocus PvuⅡ(rs2234693)between the high response group and the low response group and the normal group(P>0.05).There was no significant difference in the frequency of ERαgene locus XbaⅠ(rs9340799)between the high response group and the low response group and the normal group(P>0.05).There was no significant difference in the allele frequency of ERαlocus XbaⅠ(rs9340799)between the high response group and the normal group(P>0.05).However,the allele frequency of ERαlocus XbaⅠ(rs9340799)in the low response group was significantly different from that in the normal group(P<0.01).There was no significant difference in genotype frequency and allele frequency of ERβlocus RsaⅠ(rs1256049)and AluⅠ(rs4986938)between the high response group and the low response group and the normal group(P>0.05).Conclusion:ERαlocus XbaⅠ(rs9340799)gene polymorphism is associated with low ovarian response in COH of Han women in southern Shandong.The presence of G allele at the XbaⅠ(rs9340799)locus increases the risk of ovarian responsiveness.

关 键 词：雌激素受体 卵巢反应性 汉族妇女 

分 类 号：R711.6[医药卫生—妇产科学]