过表达Tapasin树突状细胞来源外泌体诱导T细胞分化的实验研究  

An experimental study of exosomes from Tapasin-overexpressing dendritic cells to induce T lymphocytes differentiation

作　　者：吕梦娇 吴姗姗[1] 姚婷 陈小华(指导)[1] 臧国庆(指导)[1] LYU Meng-Jiao;WU Shan-Shan;YAO Ting;CHEN Xiao-Hua;ZANG Guo-Qing(Department of Infections,the Sixth Peoples′Hospital Affiliated to Shanghai Jiaotong University,Shanghai 200233,China)

机构地区：[1]上海交通大学附属第六人民医院感染科,上海200233

出　　处：《中国免疫学杂志》2020年第22期2689-2692,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金(81770589);上海市科学技术委员会项目(17ZR1421500)。

摘　　要：目的:探讨过表达Tapasin的小鼠骨髓树突状细胞(DCs)来源外泌体在诱导小鼠脾脏T淋巴细胞分化中的作用。方法:构建过表达Tapasin病毒转染至DCs,流式细胞术鉴定CD11c和GFP表达,超高速离心法分离DCs来源外泌体(Dexs)和转染上述病毒的DCs来源外泌体(Tap-Dexs),电镜、粒径分析和Western blot鉴定,并与T淋巴细胞共培养,CCK8法和ELISA法分别检测T淋巴细胞增殖和分泌IFN-γ和IL-2水平。结果:与Dexs相比,Tap-Dexs能显著促进T淋巴细胞增殖、Th1型细胞因子IFN-γ和IL-2分泌。结论:Tap-Dexs能促进小鼠脾脏T淋巴细胞增殖和诱导T细胞分化。Objective:To investigate the role of exosomes from mouse bone marrow dendritic cells(DCs)overexpressing Tapasin in inducing splenic T lymphocytes differentiation in mice.Methods:Virus overexpressing Tapasin was constructed and transfected into DCs.CD11c antibody and GFP expressions were detected by flow cytometry,and isolated DCs-derived exosomes(Dexs)and DCs transfected with the above-mentioned virus derived exosomes(Tap-Dexs)was detected by ultracentrifugation.Electron microscopy,nanoparticle tracking analysis and Western blot were used for exosomes identification and co-cultured with T lymphocytes.CCK8 and ELISA methods were respectively used to detect T lymphocyte proliferation and secretion of IFN-γand IL-2.Results:Compared with Dexs,Tap-Dexs can promote T lymphocytes proliferation and secretion of Th1 cytokines IFN-γand IL-2 significantly.Conclusion:Tap-Dexs can promote proliferation and differentiation of spleen T lymphocytes in mice.

关 键 词：TAPASIN 树突状细胞 外泌体 T细胞分化 

分 类 号：R392[医药卫生—免疫学]