蒙农红豆草花瓣转录组微卫星SSR特征分析  

SSR characteristics of transcriptome of Mengnong sainfoin

作　　者：乔雨 石凤翎 张雨桐 钱亚斯 闫伟 伊风艳[2] QIAO Yu;SHI Fengling;ZHANG Yutong;QIAN Yasi;YAN Wei;YI Fengyan(College of Grassland,Resources and Environment,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010019,China;Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences,Inner Mongolia Prataculture Research Center,Chinese Academy of Sciences,Hohhot,Inner Mongolia 010031,China)

机构地区：[1]内蒙古农业大学草原与资源环境学院,草地资源教育部重点实验室,内蒙古呼和浩特010019 [2]内蒙古自治区农牧业科学院,中国科学院内蒙古草业研究中心,内蒙古呼和浩特010031

出　　处：《西北农林科技大学学报（自然科学版）》2020年第12期9-17,共9页Journal of Northwest A&F University(Natural Science Edition)

基　　金：内蒙古自治区草原英才创新团队项目“内蒙古高原乡土草种质资源遗传改良与利用”;内蒙古科技计划项目“抗逆乡土草品种培育及良繁技术研究与示范”。

摘　　要：【目的】对蒙农红豆草花瓣转录组微卫星SSR特征进行分析,为开发新的分子标记及探明各类性状的遗传机制研究奠定基础。【方法】以蒙农红豆草突变群体中浅色花瓣和紫红色花瓣(对照)为试验材料,通过Illumina HiSeqTM 2000对其进行高通量测序和组装,利用MISA软件对其所有Unigene进行SSR搜索,并对SSR分布及其序列特征进行分析。【结果】在蒙农红豆草转录组数据中共获得53009条Unigene,其中6202条Unigene上分布了8175个SSR位点,每5.86 kb就有1个SSR位点;重复基元数量以三核苷酸为主(49.13%),二核苷酸次之(22.07%);在284种重复基元中以AG/CT类型最高(16.16%),其次为AAG/CTT(14.30%);三核苷酸基元重复次数种类最多(11种),二核苷酸次之(10种)。随着基元重复次数的增加,核苷酸数量减少。蒙农红豆草SSR长度为12~441 bp,主要集中在12~24 bp,其中具有高度多态性潜能的SSR(长度≥20 bp)有3130条(38.29%)。【结论】蒙农红豆草SSR位点出现频率高、基元类型丰富、分布密度大、多态性潜能高,在红豆草基因定位和分子标记辅助育种等方面具有较大的应用价值。【Objective】This study analyzed SSR characteristics of petal transcriptome of Mengnong sainfoin to provide basis for development of new molecular markers and genetic mechanism of various traits.【Method】Light color flowers from mutant population and purplish-red flowers in the control group of Mengnong sainfoin were selected and high-throughput sequencing of petals was conducted with Illumina HiSeqTM 2000 and de novo.Then,SSR from all Unigenes were searched using software MISA and SSR characteristics were analyzed.【Result】A total of 53009 Unigene were obtained from the transcriptome of Mengnong sainfoin petals,and 8175 SSR loci were distributed on 6202 Unigene with a distribution density of 5.86 kb.The numbers of repeat motifs were mainly tri-nucleotide(49.13%),followed by di-nucleotide(22.07%).AG/CT type was the highest(16.16%)among 284 repeat motifs,followed by AAG/CTT(14.30%).Tri-nucleotide type of motifs repeated the most,followed by di-nucleotide.The number of nucleotides decreased with the increase of motif repeats.The length of SSRs of Mengnong sainfoin was 12-441 bp with the most in 12-24 bp.There were 3130 SSR showing high polymorphism potential with length greater than or equal to 20 bp,accounting for 38.29%of total SSRs.【Conclusion】The SSR locus in the petal transcriptome of Mengnong sainfoin had high occurring frequency,motif type richness,high distribution density and high polymorphism potential,and these characteristics can be applied in future studies.

关 键 词：蒙农红豆草 花瓣转录组 微卫星SSR 

分 类 号：S541.4[农业科学—作物学]