子痫前期患者胎盘中E2F1的表达及其对滋养细胞增殖的调控作用  

Expression of transcription factor E2F1 in placenta of preeclampsia patients and its regulation on trophoblast proliferation

作　　者：李静[1] 张佳琦[1] 许佳 李泽飞 逯敏敏 LI Jing;ZHANG Jia-qi;XU Jia(Department of Outpatient,the First Affiliated Hospital of Hebei North University,Zhangjiakou Hebei 075000,China)

机构地区：[1]河北北方学院附属第一医院门诊部,河北张家口075000

出　　处：《临床和实验医学杂志》2020年第23期2507-2510,共4页Journal of Clinical and Experimental Medicine

基　　金：河北省卫生健康委员会项目(编号:20200512)。

摘　　要：目的探究子痫前期(PE)患者胎盘中转录因子E2F1的表达及其对滋养细胞增殖的调控作用。方法收集PE胎盘标本和正常胎盘标本,检测E2F1的信使RNA表达水平。培养HTR8/Svneo细胞,转染E2F1及阴性对照(NC)的小干扰RNA(siRNA)后测定细胞周期比率及细胞周期蛋白[细胞周期蛋白B1(Cyclin B1)、细胞周期蛋白E(Cyclin E)、细胞周期蛋白依赖性激酶2(CDK2)]、凋亡基因[B淋巴细胞瘤-2基因(B-cell lymphoma-2,Bcl-2)、Bcl-2相关X蛋白(Bax)、自杀相关因子(Fas)、Fas配体(FasL)]的信使RNA表达水平。结果PE胎盘组织中E2F1的信使RNA表达水平为(0.65±0.09),明显低于对照组(1.07±0.22),差异有统计学意义(P<0.05)。E2F1-siRNA组细胞中E2F1的mRNA表达水平为(0.45±0.08),明显低于NC-siRNA组细胞中(1.02±0.27),差异有统计学意义(P<0.05)。E2F1-siRNA组细胞的G0/G1期、S期、G2/M期比率分别为(36.53±7.31)%、(33.95±6.51)%、(29.52±5.58)%,对照组为(54.42±8.83)%、(25.41±4.42)%、(20.17±2.68)%。E2F1-siRNA组细胞中Cyclin B1、Cyclin E、CDK2、Bcl-2、Bax、Fas、FasL的信使RNA表达水平分别为0.54±0.07、0.71±0.08、0.42±0.08、0.67±0.08、1.52±0.18、1.49±0.22、1.73±0.29,对照组为1.02±0.17、1.01±0.14、0.98±0.15、1.04±0.17、1.02±0.15、0.97±0.14、1.05±0.18。E2F1-siRNA组细胞的G0/G1期比率及Bax、Fas、FasL的信使RNA表达水平均明显高于NC-siRNA组,S期、G2/M期比率及Cyclin B1、Cyclin E、CDK2、Bcl-2的信使RNA表达水平均明显低于NC-siRNA组,差异有统计学意义(P<0.05)。结论子痫前期胎盘中转录因子E2F1的低表达可使滋养细胞的细胞周期发生停滞,这可能与疾病的发生密切相关。Objective To study the expression of transcription factor E2F1 in placenta of preeclampsia patients and its regulation on trophoblast proliferation.Methods PE placenta and normal placenta were collected and the mRNA expression of E2F1 were detectd.HTR8/Svneo cells were cultured and transfected with siRNA of E2F1 and negative control(NC)to determine the cell cycle ratio and the expression level of cyclin[Cyclin B1(Cyclin B1),Cyclin E(Cyclin E),Cyclin-dependent kinase 2(CDK2)]and apoptotic gene[B-cell lymphoma-2 gene(B-cell lymphoma-2,Bcl-2),Bcl-2 related X protein(Bax),suicide related factor(Fas),Fas ligand(FasL)].Results The messenger RNA expression level of E2F1 in PE placenta tissue was(0.65±0.09),which was significantly lower than that of the control group(1.07±0.22),and the difference was statistically significant(P<0.05).The mRNA expression level of E2F1 in the E2F1-siRNA group was(0.45±0.08),which was significantly lower than that in the NC-siRNA group(1.02±0.27),and the difference was statistically significant(P<0.05).The ratios of G0/G1,S,and G2/M phases of the E2F1-siRNA group were(36.53±7.31)%,(33.95±6.51)%,(29.52±5.58)%,respectively,and the control group was(54.42±8.83)%,(25.41±4.42)%,(20.17±2.68)%.The messenger RNA expression levels of Cyclin B1,Cyclin E,CDK2,Bcl-2,Bax,Fas,FasL in E2F1-siRNA group cells were 0.54±0.07,0.71±0.08,0.42±0.08,0.67±0.08,1.52±0.18,1.49±0.22,1.73±0.29,the control group was 1.02±0.17,1.01±0.14,0.98±0.15,1.04±0.17,1.02±0.15,0.97±0.14,1.05±0.18.The G0/G1 phase ratio and the messenger RNA expression levels of Bax,Fas and FasL in the E2F1-siRNA group were significantly higher than those in the NC-siRNA group.The ratio of S phase and G2/M phase and Cyclin B1,Cyclin E,CDK2,Bcl-2 The messenger RNA expression levels of were significantly lower than those of the NC-siRNA group,and the differences were statistically significant(P<0.05).Conclusion Low expression of transcription factor E2F1 in placenta of preeclampsia can arrest the cell cycle of trophoblasts,whic

关 键 词：子痫前期 E2F1 细胞周期 增殖 凋亡 

分 类 号：R714.244[医药卫生—妇产科学]