生长抑素和皮质抑素双表达DNA疫苗免疫杂交水牛犊牛的促生长效果研究  

Study on the Growth Promoting Effect of Crossbred Buffalo Calves Immunized with DNA Vaccine of Co-expression of Somatostatin and Cortistatin

作　　者：杨帅 胡祥维 周迪 张欣欣 杨利国[1] YANG Shuai;HU Xiangwei;ZHOU Di;ZHANG Xinxin;YANG Liguo(College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学动物科技学院,武汉430070

出　　处：《中国畜牧兽医》2020年第12期4041-4050,共10页China Animal Husbandry & Veterinary Medicine

基　　金：奶牛繁育技术创新与应用(2662019PY014);国家现代农业体系技术专项(CARS-36)。

摘　　要：试验旨在探究生长抑素(SS)和皮质抑素(CST)双表达DNA疫苗免疫杂交水牛犊牛的促生长效果及疫苗本身的安全性。将鉴定正确的pVGS/2SS-2A-S/CST-asd质粒电转入减毒猪霍乱沙门氏菌C500构建SS和CST双表达活载体疫苗,喷鼻免疫杂交水牛犊牛后观察其对犊牛生长性能的影响。选取体重相近的2~6月龄杂交水牛犊公牛24头,随机分为低(TL)、中(TM)、高(TH)剂量组和PBS对照组(NC),每组6头,初次免疫(简称初免)持续3 d,间隔2周后用相同剂量加免一次。结果显示,初免后2周便可产生抗SS和CST抗体,以TL组最佳,表现为更高的抗体水平和阳性率,7周时各试验组阳性率呈下降趋势;TL和TM组的平均日增重在初免后2和7周均高于对照组,但各组间差异均不显著(P>0.05),而抗体阳性组(P)的平均日增重在0~2周显著高于阴性组(N)(P<0.05)。相关激素的检测结果显示,在初免后2和7周,试验组生长激素(GH)和胰岛素生长因子1(IGF-1)的水平均高于对照组,且以TL组表现最佳,除初免后7周GH与对照组无显著差异外,其余均极显著高于对照组(P<0.01);初免后2周抗体阳性组极显著高于阴性组(P<0.05)。细胞因子检测结果显示,在初免后2周,试验组白细胞介素4(IL-4)含量高于对照组,且TL和TM组均极显著高于对照组(P<0.01),尤以TL组最高,免疫后7周TL和TH组均显著高于对照组(P<0.05),而免疫后2周对照组免疫干扰因子γ(INF-γ)含量极显著高于试验组(P<0.01),免疫后7周对照组INF-γ含量显著低于TL和TH组(P<0.05);阳性组IL-4水平在免疫后2周、INF-γ水平在免疫后7周均显著高于阴性组(P<0.05),其他各组间差异不显著(P>0.05)。对血清中血糖(GLU)、总蛋白(TP)、总胆固醇(CH)和尿素氮(BUN)水平进行测定发现,除免疫后2周总蛋白含量TL组显著高于对照组外(P<0.05),其他指标各组间差异均不显著(P>0.05)。对免疫后的水样、土样和粪样进行目的基因扩增发现,在PCR灵敏度The purpose of this study was to investigate the growth promoting effect and the safety of the vaccine in crossbred buffalo calves immunized with somatostatin and corticostatin co-expression DNA vaccine.The identified plasmid of pVGS/2SS-2A-S/CST-asd was electroporated into attenuated Salmonella Choleraesuis C500 to construct the co-expression live vector vaccine of somatostatin and corticostatin,and evaluate the effect on the growth performance of the crossbreds buffalo after nasal immunization.Twenty four 2-6 months of age crossbred buffalo calves with similar body weight were randomly divided into 4 groups immunized with the vaccine in low dosage group(TL),middle dosage group(TM),high dosage group(TH)and negative control group(NC).Immunization was performed once a day for 3 consecutive days,and a booster was given 2 weeks late.The results showed that the antibody of SS and CST could be produced on 2 weeks after the primary immunization,and TL group was the best,showing higher antibody level and positive rate.At 7 weeks,the positive rate of experimental group showed a downward trend.The daily gain of each experimental group was higher than that of control group at 2 and 7 weeks after the primary immunization,but there was no significant difference between experimental groups(P>0.05),while the average daily gain of antibody positive group(P)was significantly higher than that of negative group(N)at 2 weeks(P<0.05).The results of related hormones showed that the levels of growth hormone(GH)and insulin growth factor 1(IGF-1)were higher in experimental groups at 2 and 7 weeks after the primary immunization.In each experimental group,TL group showed the best performance,both extremely significantly higher than that of control group except GH level at 7 weeks after the primary immunization(P<0.01),antibody positive group was also significantly higher than negative group(P<0.05).Cytokine test results showed that the interleukin 4(IL-4)level in experimental groups at 2 weeks after the primary immunization was higher tha

关 键 词：生长抑素 皮质抑素 基因免疫 水牛犊牛 生长性能 安全性 

分 类 号：S823.9[农业科学—畜牧学]