北京市伴侣动物源肠球菌的抗菌药物耐受情况调查  

Research on Antimicrobial Resistance Characteristics of Enterococcus spp.from Companion Animals in Beijing

作　　者：陈霞[1] 赵晓菲[1] 白雪梅[1] 车洁[1] 张云飞 袁敏[1] 李娟[1] CHEN Xia;ZHAO Xiaofei;BAI Xuemei;CHE Jie;ZHANG Yunfei;YUAN Min;LI Juan(State Key Laboratory for Infectious Disease Prevention and Control,Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,感染性疾病诊治协同创新中心,北京102206

出　　处：《中国畜牧兽医》2020年第12期4119-4126,共8页China Animal Husbandry & Veterinary Medicine

基　　金：“十三五”国家科技重大专项“病原菌耐药性检测、监测关键技术及传播规律研究”(2018XZ10733-402)。

摘　　要：为研究北京市伴侣动物源肠球菌对常见抗菌药物的耐受情况,于2015年5月至2016年1月以北京市某宠物医院门诊就诊犬、猫作为采样对象,采集犬、猫自然排出的粪便作为样品,共得到样本320份。采用选择性培养基进行菌株分离和VITEK-2型全自动微生物分析仪进行肠球菌的鉴定;对分离鉴定的菌株进行8类11种常见抗菌药物敏感性试验;同时进行高水平庆大霉素和高水平链霉素耐受的检测试验,对受试肠球菌的耐药性和多重耐药性进行分析。共得到菌落形态及生化特性不同的非重复的肠球菌318株,其中粪肠球菌(49.06%)和屎肠球菌(29.87%)分离率较高。受试肠球菌对高水平庆大霉素和高水平链霉素的耐药率分别为39.94%和43.40%,对四环素(78.62%)、红霉素(67.30%)和奎诺普丁-达福普汀耐药率(43.71%)较高,对替加环素全部敏感,对万古霉素和呋喃妥因非耐药率也很高(均为98.74%)。受试肠球菌对1类到7类抗菌药物有不同程度的耐药性,多重耐药现象普遍,多重耐药率高达57.23%。耐受4类抗菌药物的数量最多(n=65株)。经分析共得到44种不同的耐药谱,以耐受4类药物的红霉素-奎诺普丁-达福普汀-四环素-高水平氨基糖苷类耐药谱占比最高(45/318,14.15%)。本研究证实受试的北京市伴侣动物源肠球菌对常见抗菌药物的耐药程度较高,多重耐药情况普遍存在,应加强对其耐药性的监测工作。In order to study the tolerance of Enterococcus spp.from companion animals to common antibiotics in Beijing,320 samples were collected from dogs and cats in a pet hospital in Beijing from May 2015 to January 2016.The strains were isolated by selective culture medium and identified by VITEK-2 Compact 60.The sensitivity test of 11 kinds of common antibiotics in 8 categories was carried out for the isolated and identified strains.At the same time,the high-level gentamicin and high-level streptomycin resistance test were carried out to analyze the drug resistance and multiple drug resistance of Enterococcus.A total of 318 strains of non repetitive enterococci with different colony morphology and biochemical characteristics were obtained.The isolation rates of Enterococcus faecalis(49.06%)and Enterococcus faecium(29.87%)were higher.The resistance rates of Enterococcus to high-level gentamicin and high-level streptomycin were 39.94%and 43.40%,respectively.The resistance rates to tetracycline(78.62%),erythromycin(67.30%)and quinoproptin dapfoptin(43.71%)were very high.All of them were sensitive to tigecycline,and the non-resistant rate to vancomycin and nitrofurantoin was also high(98.74%).Enterococcus tested showed different degrees of resistance to class 1 to class 7 antibiotics,and the multidrug resistance was common,and the multidrug resistance rate was as high as 57.23%.The number of resistant antibiotics was the highest(n=65).A total of 44 different drug resistance profiles were obtained.Among them,erythromycin-quinoptin-daptoptin-tetracycline-high level aminoglycoside resistant spectrum accounted for the highest proportion(45/318,14.15%).This study confirmed that Enterococcus spp.isolated from companion animals in Beijing was highly resistant to common antibiotics,and multidrug resistance was prevalent.Therefore,it was necessary to strengthen the monitoring of drug resistance.

关 键 词：伴侣动物 肠球菌 耐药性 多重耐药性 

分 类 号：S852.611[农业科学—基础兽医学]