海洋小单孢菌株产抗肿瘤新化合物FW05-0328-1的诱变选育及发酵工艺研究  

Mutagenesis breeding and fermentation process optimization of FW05-0328-1-a macrolactam compound derived from marine Micromonospora with antitumor activities

作　　者：孙菲[1] Sun Fei(Fujian Key Laboratory of Screening for Novel Microbial Products,Fujian Institute of Microbiology,Fuzhou 350007)

机构地区：[1]福建省微生物研究所,福建省新药(微生物)筛选重点实验室,福州350007

出　　处：《中国抗生素杂志》2020年第10期989-993,共5页Chinese Journal of Antibiotics

基　　金：国家重点研发计划(No.2018YFC0311004);福建省公益类科研院所省重点项目(No.2019R1005-1)。

摘　　要：目的获得抗肿瘤化合物FW05-0328-1高产菌株,提高其发酵产量。方法采用紫外(UV)及常压室温等离子体技术(ARTP)复合诱变筛选化合物FW05-0328-1高产菌株,结合高产菌株摇瓶发酵特性,优化100L全自动发酵罐发酵工艺。结果获得1株高产FW05-0328-1菌株Micromonospora sp.FIM0O123,其在100L全自动发酵罐发酵效价达187.3mg/L,较原始菌株提高了17倍。结论高产菌株的筛选及100L全自动发酵罐发酵工艺优化能有效提高化合物FW05-0328-1产生能力。Objective To obtain a high producing strain of a novel anti-tumor compound FW05-0328-1 and improve its production.Methods Multi-treatment of ARTP,UV was employed to obtain a high FW05-0328-1-producing strain.The fermentation process of Micromonospora sp.FIM0O123 was optimized on 100L fermentor according to its fermentation characteristics in shake flask.Results Micromonospora sp.FIM0O123-a high FW05-0328-1-producing strain was obtained,the potency of the mutant could reach as high as 187.3mg/L on 100L fermentor,which is 17 times higher than that of the original strain.Conclusion Through screening of high FW05-0328-1-producing strain combining with its fermentation process optimization on 100L fermentor,the production level of compound FW05-0328-1 was inhanced siginificantly.

关 键 词：化合物FW05-0328-1 抗肿瘤 诱变 100L发酵罐 

分 类 号：R979.1[医药卫生—药品]