甲基营养型芽孢杆菌BMF04菌株的抑菌谱及对仁果类真菌病害的防治作用  

The Inhibitory Spectrum Determination of Bacillus methylotrophics BMF 04 and Control Effect on Fungal Disease in Kernel Fruits

作　　者：万丹丹 吴海霞[1] 汤曼丽 彭云 陈茹 马桂珍 暴增海 WAN Dandan;WU Haixia;TANG Manli;PENG Yun;CHEN Ru;MA Guizhen;BAO Zenghai(School of Marine Science and Fisheries,Jiangsu Ocean University,Jiangsu Lianyungang 222000,China;Kangda College of Nangjing Medical University,Jiangsu Lianyungang 222000,China)

机构地区：[1]江苏海洋大学海洋科学与水产学院,江苏连云港222000 [2]南京医科大学康达学院,江苏连云港222000

出　　处：《现代农药》2020年第6期45-48,54,共5页MODERN AGROCHEMICALS

基　　金：农业科技自主创新资金项目(CX(20)3133);南京医科大学康达学院2018年度科研发展基金一般项目(KD2018KYJJYB015)。

摘　　要：为了探究海洋甲基营养型芽孢杆菌BMF 04菌株的抑菌谱及其对仁果类真菌病害的防效,采用平板对峙法和牛津杯法测定BMF 04菌株及其无菌发酵液对26种植物病原真菌和5种细菌的抑菌活性;采用室内保湿法测定该菌株发酵液和无菌发酵液在果实上对苹果轮纹病菌的防治作用。结果表明,BMF 04菌株及其发酵液对供试的26种植物病原真菌和2种细菌均具有抑制作用;该菌株的发酵液和无菌发酵液对苹果和梨的轮纹病均具有防治作用,且保护效果优于治疗效果,最高防效分别为76.47%、51.77%,说明BMF 04是一株具有广谱抗菌性的海洋细菌,对果实轮纹病具有良好的防效,具有开发微生物制剂的潜力。In order to investigate the inhibitory spectrum of Bacillus methylotrophics BMF 04 strain and its control effect on nut fungus disease,we used plate stand-off method and Oxford cup method to determine the bacteriostatic activity of fermentation broth and aseptic cell-free fermentation broth of the BMF 04 strain against 26 kinds of plant pathogenic fungus and 5 kinds of bacteria.The effect of fermentation broth and cell-free fermentation broth of the strain on the control effect of Botryosphaeria dothidea was measured by the indoor moisturizing method.The results showed that BMF 04 strain and its fermentation broth had inhibitory effects on 26 kinds of plant pathogenic fungus and 2 kinds of bacteria.The fermentation broth and cell-free fermentation broth of the strain can prevent and cure apple and pear's ring rot,and the protective effect was better than the therapeutic effect,the highest control effect value was 76.47%,51.77%,respectivedly.The above results suggest that BMF 04 is a marine bacterium with broad-spectrum antimicrobial activity,which has good control effect on fruits'ring rot and has the potential to be develpoed as a microbial agent.

关 键 词：甲基营养型芽孢杆菌 BMF 04菌株 抑菌谱 苹果轮纹病菌 防效 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治] S432.44[农业科学—植物保护]