人参皂苷F2干预NF-κB通路抑制H2O2诱导的细胞凋亡  

Ginsenoside F2 Intervenes the NF-κB Pathway to Inhibit H2O2-induced Apoptosis

作　　者：刘迪 于子翔 张浩 张寒雪 孔繁利 冯宪敏 LIU Di;YU Zi-xiang;ZHANG Hao;ZHANG Han-xue;KONG Fan-li;FENG Xian-min(College of Basic Medicine,Jilin Medical University,Jilin 132013,China;College of Medical Technology,Beihua University,Jilin 132013,China)

机构地区：[1]吉林医药学院基础医学院,吉林吉林132013 [2]北华大学医学技术学院,吉林吉林132013

出　　处：《现代食品科技》2020年第12期1-6,共6页Modern Food Science and Technology

基　　金：吉林省卫生健康科技能力提升计划项目(2019Q031);吉林省教育厅科学研究规划项目(JJKH20180352KJ)。

摘　　要：本研究通过测定人参皂苷F2对凋亡细胞乳酸脱氢酶(LDH)释放率和线粒体膜电位的影响,运用q RT-PCR和Western blot检测F2对NF-κB凋亡信号通路中p65蛋白的影响。发现H2O2损伤细胞的LDH释放率较对照组的有明显提高42.72%;线粒体膜电位下降,损伤组的相对荧光值较对照组降低44.03%;NF-κB p65的蛋白和mRNA表达量分别为122.73%、1.66,明显高于对照组(p<0.05)。1.25、5、20μmol/LF2预处理损伤细胞后,LDH释放率较损伤组显著降低(p<0.05),分别降至82.71%、75.69%、69.61%;线粒体膜电位较损伤组显著提高,不同浓度F2的相对荧光值分别为:60.22%、62.76%、70.96%,(p<0.05);随着F2浓度的升高,NF-κB通路中p65的蛋白表达分别下降到79.49%、71.92%、58.39%,(p<0.05),mRNA表达下调至1.30、1.18、1.01,(p<0.05)。结果表明,人参皂苷F2能通过降低凋亡细胞的LDH释放率、升高线粒体膜电位、抑制NF-κB信号通路激活发挥抗凋亡作用,为人参皂苷抗氧化应激诱导的细胞凋亡提供实验依据。In this study,the effects of ginsenoside F2 on p65 protein in the NF-κB apoptosis signaling pathway were examined by q RT-PCR and Western blot techniques,and through measuring the effects of ginsenoside F2 on the release rate of lactate dehydrogenase(LDH)and and mitochondrial membrane potential(MMP)of apoptotic cells.It was found that the H2O2 injured cells had a significantly increased LDH release rate(by 42.72%),decreased mitochondrial membrane potential,and decreased relative fluorescence value(by 44.03%),and increased protein and mRNA expression of NF-κB p65(as 122.73%and 1.66,respectively),compared with the control group(p<0.05).After pretreatment of cells with 1.25,5,and 20μmol/L F2,the LDH release rates of the injured group were significantly lowered(p<0.05),to 82.71%,75.69%,and 69.61%respectively,while the mitochondrial membrane potential values were significantly increased(p<0.05)(with the corresponding relative fluorescence values as 60.22%,62.76%and 70.96%,respectively).With the increase of F2 concentration,the protein expression of p65 in the NF-κB pathway decreased to 79.49%,71.92%and 58.39%,respectively(p<0.05),and the mRNA expression was down-regulated to 1.30,1.18 and 1.01,respectively(p<0.05).The results showed that ginsenoside F2 exhibited an anti-apoptotic effect though reducing the LDH release rate of apoptotic cells,increasing the mitochondrial membrane potential,and inhibiting the activation of NF-κB signaling pathway.This study provides an experimental basis for suppressing effect of ginsenosides on oxidative stress-induced apoptosis.

关 键 词：人参皂苷 抗凋亡 人胚肾细胞(HEK-293细胞) 人参 

分 类 号：R285[医药卫生—中药学]