表达Cry1Ab和Cry2Ab蛋白的转基因玉米GAB-3对四种主要鳞翅目害虫的抗性评价  

Resistance Evaluation of Genetically Modified Maize GAB-3 Expressing Cry1Ab and Cry2Ab Against Four Major Lepidopteran Pests

作　　者：徐晓丽[1] 姜媛媛 王鹏飞 来勇敏 陈笑芸[1] 徐俊锋[1] XU Xiaoli;JIANG Yuanyuan;WANG Pengfei;LAI Yongmin;CHEN Xiaoyun;XU Junfeng(Institute of Agro-product Safty and Nutrition,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310012,China)

机构地区：[1]浙江省农业科学院农产品质量安全与营养研究所,杭州310021 [2]浙江大学农业与生物技术学院,杭州310012

出　　处：《中国农业科技导报》2020年第12期97-104,共8页Journal of Agricultural Science and Technology

基　　金：浙江省公益技术应用研究计划项目(2019R19A52C02)。

摘　　要：为评价表达Cry1Ab和Cry2Ab蛋白的转基因玉米GAB-3对黏虫Mythimna separata(Walker)、亚洲玉米螟Ostrinia furnacalis(Guenée)、棉铃虫Helicoverpa armigera(Hübner)和草地贪夜蛾Spodoptera frugiperda(J. E. Smith)的杀虫效果,采用室内生测的方法研究了GAB-3不同组织器官对以上四种害虫存活率的影响,并通过田间接虫试验进一步测试了GAB-3对黏虫和亚洲玉米螟的抗性。室内生测结果显示:转基因玉米GAB-3心叶期叶片对黏虫、亚洲玉米螟、棉铃虫和草地贪夜蛾具有很强的毒杀作用,苞叶、穗尖、穗轴、花丝、雄穗和籽粒对亚洲玉米螟和棉铃虫具有显著的抗性。田间接虫试验结果显示:心叶期GAB-3高抗黏虫和亚洲玉米螟,穗期GAB-3高抗亚洲玉米螟。上述结果表明,转基因玉米GAB-3对4种主要玉米鳞翅目害虫有很好的杀虫效果,具有较好的商业化应用前景。In order to evaluate the resistance of GAB-3 expressing Cry1 Ab and Cry2 Ab against four lepidopteran insect pests, Mythimna separata(Walker), Ostrinia furnacalis(Guenée), Helicoverpa armigera(Hübner) and Spodoptera frugiperda(J. E. Smith), the survival of the larvae of the four insect pests treated with GAB-3 were studied by laboratory bioassays. A field study was also conducted to study the control efficacy of GAB-3 to M. separata and O. furnacalis. The results of laboratory bioassays showed that the whorl leaves of GAB-3 had strong toxicity to M. separata, O. furnacalis, H. armigera and S. frugiperda. The husks, ear tips, cobs, silks, tassels and kernels of GAB-3 showed significantly insecticidal efficacy against O. furnacalis and H. armigera. Damage ratings at whorls and ears in the field trials also showed that GAB-3 was highly resistant to M. separata and O. furnacalis in whorl stage and to O. furnacalis in ear stage. Above results showed that GAB-3 had good potential for commercial application for its significantly high resistance to four major lepidopteran pests of corn.

关 键 词：转基因玉米GAB-3 鳞翅目害虫 室内生测 抗虫性 

分 类 号：Q78[生物学—分子生物学]