可注射海藻酸钠/泊洛沙姆复合水凝胶的制备及药物缓释研究  

Preparation of injectable sodium alginate/poloxamer composite hydrogel for sustained drug release

作　　者：候冰娜 郑泽邻 赵梓年 李进 倪凯 赵琳琳[3] 李征征 HOU Bing-na;ZHENG Ze-lin;ZHAO Zi-nian;LI Jin;NI Kai;ZHAO Lin-lin;LI Zheng-zheng(College of Chemical Engineering and Materials,Tianjin University of Science and Technology,Tianjin 300457,China;Nanjing Linhou Environmental Protection Technology Co.,Ltd.,Nanjing 210001,China;School of Materials Science and Engineering,Tianjin University of Technology,Tianjin 300457,China;Tianjin Key Laboratory of Pulp and Paper,Tianjin University of Science and Technology,Tianjin 300457,China;State Key Laboratory of Molecular Engineering of Polymers,Fudan University,Shanghai 200433,China)

机构地区：[1]天津科技大学化工与材料学院,天津300457 [2]南京霖厚环保科技有限公司,南京210001 [3]天津理工大学材料科学与工程学院,天津300457 [4]天津科技大学天津市制浆造纸重点实验室,天津300457 [5]复旦大学聚合物分子工程国家重点实验室,上海200433

出　　处：《材料工程》2020年第12期60-67,共8页Journal of Materials Engineering

基　　金：2018年度天津市教委科研计划项目(2018KJ110);天津市海洋资源与化学重点实验室(201706);天津市制浆造纸重点实验室开放基金资助项目(201809);国家自然科学基金资助项目(51703163)。

摘　　要：泊洛沙姆是一种温敏性合成聚合物,随着温度改变能够实现溶胶-凝胶转变,但其相对分子质量低,水凝胶结构很难长期保持。本研究以泊洛沙姆为基体,通过与海藻酸钠溶液混合制备了温敏性海藻酸钠/泊洛沙姆复合水凝胶(SA/P 407)。通过傅里叶变换红外光谱(FT-IR)、试管倒置法、扫描电子显微镜(SEM)、旋转流变仪和紫外-可见分光光度计(UV-Vis)对海藻酸钠/泊洛沙姆复合水凝胶的结构、温敏性、微观形态、动态黏弹性和体外药物释放性能进行了表征,此外还研究了海藻酸钠/泊洛沙姆复合水凝胶的溶胀性能。结果表明:海藻酸钠/泊洛沙姆复合水凝胶具有温敏性,通过加入海藻酸钠能够降低泊洛沙姆在体温下的成胶浓度(质量分数为6%);通过控制海藻酸钠与泊洛沙姆的质量比,能够使溶胶-凝胶转变温度处于室温与体温(25~37℃)之间,并且缩短凝胶化时间为84 s;海藻酸钠/泊洛沙姆复合水凝胶具有高度孔隙化且孔隙之间相互连通的结构特点,其孔径大小处于20~80μm范围内;随着海藻酸钠添加量的增加,海藻酸钠/泊洛沙姆复合水凝胶的溶胀率逐渐降低;海藻酸钠/泊洛沙姆复合水凝胶对抗癌药物吉西他滨具有缓释作用,药物释放时间可达72 h。海藻酸钠/泊洛沙姆复合水凝胶在可注射药物缓释载体方面具有重要的应用前景。Poloxamer is a thermo-sensitive synthetic polymer that can achieve sol-gel transition with temperature change,but its relative molecular mass is low,and the hydrogel structure is difficult to maintain for a long time.The thermo-sensitive sodium alginate/poloxamer composite hydrogel(SA/P 407)was synthesized by mixing poloxamer with sodium alginate.The chemical structures,temperature sensitivity,microscopic morphology,dynamic viscoelasticity and in vitro drug release behaviors of sodium alginate/poloxamer composite hydrogels were investigated by FT-IR,test tube inversion,SEM,rheometer and UV-Vis spectroscopy.In addition,the swelling properties of sodium alginate/poloxamer composite hydrogels were also studied.These results show that the sodium alginate/poloxamer composite hydrogel is thermo-sensitive,and the gelation concentration(mass fraction is 6%)of poloxamer at body temperature can be reduced by adding sodium alginate.By controlling the mass ratio of sodium alginate and poloxamer,the sol-gel transition temperature can be kept between room temperature and body temperature(25-37℃),and the gelation time can be shortened to 84 s.The sodium alginate/poloxamer composite hydrogel has a structural feature of high porosity and interconnected pores,and its pore size ranges from 20μm to 80μm.With the increase of sodium alginate,the swelling rate of the sodium alginate/poloxamer composite hydrogel is gradually decreased.The sodium alginate/poloxamer composite hydrogel shows sustained release of the anticancer drug gemcitabine,and the drug release time can reach 72 hours.Sodium alginate/poloxamer composite hydrogel has an important application prospect in the field of injectable carriers for sustained drug release.

关 键 词：海藻酸钠 泊洛沙姆 溶胶-凝胶转变 药物缓释 

分 类 号：O63[理学—高分子化学]