副溶血弧菌TDH快速免疫胶体金检测板的研制  

Development of colloidal gold immunochromatography plate for rapid detection of TDH produced by Vibrio parahaemolyticus

作　　者：杨靖亚 方艳 陆晓帆 张建 赵勇 YANG Jing-Ya;FANG Yan;LU Xiao-Fan;ZHANG Jian;ZHAO Yong(College of Food,Shanghai Ocean University,Laboratory of Quality and Safety Risk Assessment for Aquatic Product on Storage and Preservation, Ministry of Agriculture,Shanghai 201306,China)

机构地区：[1]上海海洋大学食品学院,农业部水产品贮藏保鲜质量安全风险评估实验室,上海201306

出　　处：《中国免疫学杂志》2020年第21期2635-2639,共5页Chinese Journal of Immunology

基　　金：上海市科委工程中心能力提升项目(No.16DZ2280300)。

摘　　要：目的:开发特异性检测副溶血弧菌耐热直接溶血毒素(TDH)的快速免疫胶体金检测板。方法:利用本实验室前期制备的两株TDH单克隆抗体T6D4和T9H4,以双抗体夹心法研制胶体金检测板。结果:研制出的快速免疫胶体金检测板能够特异性地检测TDH,检测限为500 ng/ml。结论:成功研制出能够特异性检测副溶血弧菌TDH的快速免疫胶体金检测板,为TDH的快速检测提供了新的测试工具。Objective:To develop colloidal gold immunochromatographic test plate for specific detection of Vibrio parahaemolyticus heat-resistant direct hemolytic toxin(TDH).Methods:Using two TDH monoclonal antibodies T6D4 and T9H4 prepared in our laboratory,colloidal gold immunochromatographic test plate was developed based on principle of double antibody sandwich method.Results:Developed colloidal gold immunochromatographic test plate can specifically detect TDH with a minimum detection limit of 500 ng/ml.Conclusion:Colloidal gold immunochromatographic test plates were successfully prepared to detect TDH of Vibrio parahaemolyticus specifically,which providing a new testing tool for rapid detection of TDH.

关 键 词：副溶血弧菌 耐热直接溶血毒素 快速免疫胶体金检测板 

分 类 号：R392.12[医药卫生—免疫学]