质膜修复蛋白在妊娠期高血压患者不同部位的表达对患者预后的预测价值分析  

Analysis of Plasma Membrane Repair Protein Expression in Different Parts of Patients with Pregnancy-Induced Hypertension

作　　者：高迎春[1] 潘长清[1] Gao Yingchun;Pan Changqing(Department of Obstetrics and Gynecology,Mianyang Central Hospital,Mianyang,Sichuan 621099,China)

机构地区：[1]绵阳市中心医院妇产科,四川绵阳621099

出　　处：《四川医学》2020年第11期1162-1165,共4页Sichuan Medical Journal

基　　金：四川省医学会高血压疾病专项科研课题(编号:2017SHDO16)。

摘　　要：目的研究质膜修复蛋白(Dysferlin)在妊娠期高血压疾病孕妇血清、脐带血、胎盘组织及脐带等不同组织中的表达及其对疾病预后的预测价值。方法选取2017年8月至2019年2月我院收治的120例妊娠期高血压疾病孕妇。依据病情将患者分为妊娠期高血压组(40例)、轻度子痫前期组(40例)、重度子痫前期组(40例),另外选取同期足月分娩无合并症的孕妇40例为对照组。采用ELISA法检测孕妇血清、脐带血中Dysferlin表达水平;采用RT-PCR法检测孕妇胎盘与脐带组织中质膜修复蛋白m RNA表达水平。统计患者预后情况并分析Dysferlin的表达与预后的相关性。结果与对照组相比,Dysferlin在脐带血、胎盘组织中的表达随疾病进展显著降低(P<0.05);而在静脉血、脐带组织中的表达差异无统计学意义(P>0.05)。在孕妇妊娠期不良结局与预后中,产后大出血与胎儿窘迫发生率随疾病进展显著增加,并与Dysferlin表达水平呈显著负相关(P<0.05)。结论Dysferlin在妊娠期高血压疾病中可于脐带血和胎盘组织中低表达,并且表达水平随疾病严重程度显著降低,对产后大出血和胎儿窘迫等不良预后具有一定的预测价值,可供临床参考以提前预防和治疗。Objective To study plasma membrane repair protein(Dysferlin)expression in serum,umbilical cord blood,placental tissue and umbilical cord of pregnancy-induced hypertension and predictive value for disease prognosis.Methods From August 2017 to February 2019,120 pregnant women with hypertension during pregnancyin our hospitalwere included.Basedon condition,they were divided into pregnancy-induced hypertension group(40 cases),mild preeclampsia group(40 cases),severe preeclampsia group(40 cases).In addition,40 pregnant women who gave birth at full term during the same period without complications were selected as control group.ELISA method was used to detect plasma membrane repair proteinexpression in serum and cord blood.RT-PCR was used to detect plasma membrane repair protein mR NA in the placenta and umbilical cord tissues.Prognosis,and correlation between plasma membrane repair protein and prognosis were analyzed.Results Compared with control group,Dysferlin in umbilical cord blood and placenta tissues significantly decreased as disease progressed(P<0.05).However,expression difference in venous blood and umbilical cord tissue was not statistically significant(P>0.05).In adverse outcomes and prognosis,postpartum hemorrhage and fetal distress increased significantly with progression of disease,and was significantly negatively correlated with Dysferlin(P<0.05).Conclusion Dysferlin could be low expressed in umbilical cord blood and placental tissues in pregnancy-induced hypertension,and expression level decreases significantly with disease severity.It has certain predictive value for poor prognosis such as postpartum hemorrhage and fetal distress and could be used for clinical reference.

关 键 词：妊娠期高血压 质膜修复蛋白 血清 胎盘 脐带 

分 类 号：R714.24[医药卫生—妇产科学]