一种根系分泌物收集装置的研制及在烟草分析中的应用  

Development of a device for collecting root exudates and its application in tobacco analysis

作　　者：冯超[1] 张成省 张家韬 周本国 王卫民[4] 谭效磊 崔志燕 Feng Chao;Zhang Chengsheng;Zhang Jiatao;Zhou Benguo;Wang Weimin;Tan Xiaolei;Cui Zhiyan(Key Laboratory of Tobacco Pest Monitoring&Integrated Management,Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao 266101,China;Shannxi Tobacco Company,Xi'an 710000,China;Tobacco Research Institute of Anhui Academy of Agricultural Sciences,Hefei 230001,China;Zhejiang Zhongyan Industry Co.,Ltd.,Hangzhou 310000,China;Shandong Tobacco Company,Linyi 276000,China)

机构地区：[1]中国农业科学院烟草研究所烟草行业烟草病虫害监测与综合治理重点实验室,青岛266101 [2]陕西省烟草公司,西安710000 [3]安徽省农科科学院烟草研究所,合肥230001 [4]浙江中烟工业有限责任公司,杭州310000 [5]山东省烟草公司临沂市公司,临沂276000

出　　处：《分析仪器》2020年第6期56-63,共8页Analytical Instrumentation

基　　金：中国烟草总公司陕西省公司项目“陕西烟草病虫害绿色防控技术研究示范与推广应用(SXYC-KJ-2017-01)”;中国烟草总公司安徽省公司项目“安徽省烟草绿色防控重大专项(20170551024)”;中国烟草总公司山东省公司科技项目“沂蒙烟区根茎病害防控关键技术研究与示范(201906)”。

摘　　要：针对根系分泌物收集存在的问题,研制了一种不扰动根系生长、不破坏根际环境的收集装置。利用此装置收集感染/未感染烟草疫霉菌(Phytophthoranicotianae)的烟草根系分泌物,采用液相色谱-质谱联用仪(Liquid chromatograph-mass spectrometry,LC-MS)检测获得的根系分泌物,通过PLS-DA和OPLS-DA分析根系分泌物有机物的变化,获得差异性代谢物主要为有机酸、碱、烯、醇、酯、酮、醛、酰胺等物质。在感染疫霉菌后,代谢物含量发生较大变化,其中差异性显著的物质有17种。为研究根系分泌物与疫霉菌互作识别提供重要的理论依据。In order to solve the problems existing in root exudates collection,a collection device without disturbing root growth and damaging surrounding environment was developed.The tobacco root exudates infected or uninfected by Phytophthora nicotianae were collected by this device,and were analyzed by LC-MS and multivariate statistics(PLS-DA and OPLS-DA).The main metabolites were organic acids,alkali,alkenes,alcohols,esters,ketones,aldehydes,amides and so on.The content of metabolites in tobacco root exudates infected by Phytophthora nicotianae changed greatly,among which 17 compounds had significant difference.This results provide an important theoretical basis for the study of mutual recognition of root exudates and Phytophthora.

关 键 词：根系分泌物 收集装置 液相色谱质谱(LC-MS) 差异性代谢物 

分 类 号：S237[农业科学—农业机械化工程] S572[农业科学—农业工程]