桃红四物汤组方药物主要有效单体成分配伍对早期骨折愈合的影响  

Effects of Compatibility of the Main Effective Monomer Components of Medicinal Substances in Taohong Siwu Decoction on Early Fracture Healing

作　　者：杨卓 邱振南 余晓愉 周文佳 闫春晓[1] 齐新宇[1] 徐无忌[3] 熊辉[1] YANG Zhuo;QIU Zhennan;YU Xiaoyu;ZHOU Wenjia;YAN Chunxiao;QI Xinyu;XU Wuji;XIONG Hui(Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;Liuyang Hospital of Traditional Chinese Medicine,Liuyang,Hunan 410301,China;The Second Hospital of Hunan University of Chinese Medicine,Changsha,Hunan 410005,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]浏阳市中医院,湖南浏阳410301 [3]湖南中医药大学第二附属医院,湖南长沙410005

出　　处：《湖南中医药大学学报》2020年第12期1462-1467,共6页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金项目(81574005);湖南省研究生科研创新项目(CX20190547)。

摘　　要：目的研究桃红四物汤中各药物主要有效单体成分配伍对大鼠早期骨折愈合的影响及其机制。方法采用骨锯截断股骨干中段建立骨折模型,将大鼠随机分为5组:空白组(不做处理灌胃等量生理盐水)、假手术组(只切开不截断,灌胃等量生理盐水)、模型组(造模,灌胃等量生理盐水)、原方水提组(造模,灌胃原方水煎剂)、单体成分组(造模,灌胃原方主要单体成分组方)。于造模后的第4、7、10天3个时间点:卡尺测量大鼠肢体肿胀度;ELISA检测大鼠血清中VEGF的浓度;Micro-CT扫描检测大鼠骨折断端BV和BV/TV%;HE染色观察大鼠骨折早期断端病理形态变化。结果与空白组比较,模型组肢体肿胀度、VEGF浓度均明显增高,组织病理学结构存在明显差异。术后第4、7、10天与模型组比较,单体成分组与原方水提组肢体肿胀度明显下降(P<0.05),VEGF浓度、BV、BV/TV%明显升高(P<0.05)。结论(1)桃红四物汤各药物主要有效单体成分配伍能有效缩短骨折愈合时间,促进大鼠早期骨折愈合。(2)桃红四物汤各药物有效单体成分配伍与原方水煎剂对早期骨折的治疗效果基本相同。Objective To study effects and the mechanism of compatibility of the main effective monomer components of medicinal substances in Taohong Siwu Decoction on early fracture healing in rats.Methods Middle sections of femoral shafts were cut by a bone saw to establish fracture models,and the rats were randomly assigned into 5 groups:a blank group(without treatment and gavage with the same amount of normal saline),a sham operation group(incision only,without cutting off the femoral shafts;gavage with the same amount of normal saline),a model group(gavage with the same amount of normal saline after the modeling),an original prescription water extraction group(gavage with decoction of the original prescription)and a monomer component group(with a prescription of the main monomer components in the original prescription).On 3 time points of the 4th,7th and 10thdays after the modeling:a caliper was used to measure degrees of rat limb swelling;ELISA was applied to detect concentrations of VEGF in rat serum;Micro-CT scanning was applied to detect BV and BV/TV%in rat fracture ends;and HE staining was used to observe pathological morphologic changes of the early fracture ends in rats.Results Compared with the blank group,the limb swelling degree and the VEGF concentration in the model group were significantly increased,and the histopathological structure was significantly different.Compared with the model group on the 4th,7thand 10thdays after the operation,the limb swelling degrees in the monomer component group and the original prescription water extraction group were significantly decreased(P<0.05),and the concentrations of VEGF,BV and BV/TV%were significantly increased(P<0.05).Conclusion(1)The compatibility of the main effective monomer components of medicinal substances in Taohong Siwu Decoction can effectively shorten fracture healing time and promote early fracture healing in rats.(2)The treatment effects of compounds-compatibility of Taohong Siwu Decoction are quivalent to its original water extraction.

关 键 词：桃红四物汤 单体成分配伍 骨折 早期骨折愈合 

分 类 号：R289[医药卫生—方剂学]