苍耳子中抗寒物质的提取工艺研究  

Study on Extraction Technology of Cold-resistant Substances in Fructus xanthii

作　　者：熊海蓉[1] 饶桠楠 史万杰 熊远福[1] 黄敏[3] XIONG Hai-rong;RAO Ya-nan;SHI Wan-jie(Chemistry and Materials Science,Hunan Agricultural University,Changsha,Hunan 410128;Agricultural College of Yangzhou University,Yangzhou,Jiangsu 225000)

机构地区：[1]湖南农业大学化学与材料科学学院,湖南长沙410128 [2]扬州大学农学院,江苏扬州225000 [3]湖南农业大学农学院,湖南长沙410128

出　　处：《安徽农业科学》2020年第24期171-173,183,共4页Journal of Anhui Agricultural Sciences

基　　金：国家重点研发计划项目(2017YFD0301503);作物种质创新与资源利用重点实验室(省部共建国家重点实验室培育基地)科学基金开放项目(19KFXM07);湖南农业大学校级大学生研究性学习和创新性实验计划项目(xcx19116)。

摘　　要：[目的]获得苍耳子中抗寒物质的最佳提取工艺条件。[方法]以苍耳子为原料、水稻为试验对象,研究提取剂的筛选,探讨料液比、提取温度、提取时间、提取次数等因素对苍耳子中抗寒物质提取的影响,通过L 9(34)正交试验确定最佳提取条件。[结果]苍耳子中抗寒物质的最佳提取工艺条件为提取剂50%乙醇、料液比1∶9(g∶mL)、提取温度80℃、提取时间40 min、提取3次;粉体抗寒物质的产率为10.0%。[结论]该提取工艺简便、成本较低,获得的抗寒物质能显著提高秧苗游离脯氨酸含量。[Objective]To obtain optimal conditions of extraction technology of cold-resistant substances in Fructus xanthii.[Method]Using Xanthium as raw material and rice as the test object,the effects of the solid-liquid ratio,extraction temperature,extraction time and extraction times on the extraction of cold-resistant substances in Fructus xanthii were studied,and the optimal extraction conditions were determined by orthogonal experiment L 9(34).[Result]The optimal conditions of the extraction technology of cold-resistant substances in Fructus xanthii were as followed:the extractant was 50%ethanol,1∶9(g∶mL)as solid-liquid ratio,80℃as extraction temperature,40 min as extraction time,and extraction 3 times.The yield of powder cold-resistant substances was 10.0%.[Conclusion]The extraction process was simple with low cost,and the subsequent cold-resistant substances could significantly increase the content of proline in rice seedlings.

关 键 词：苍耳子 抗寒物质 提取工艺 抗寒性 水稻 

分 类 号：Q945.78[生物学—植物学] S-3[农业科学]