泡球蚴原头节与不同浓度肝癌细胞共培养的存活率研究分析  

Study on the Survival Rate of Co-culture of Echinococcus Multilocularis Protoscolex with Different Concentrations of Hepatoma Cells

作　　者：杨照辉 邓子 张宏伟[1] 褚志强[1] 张龙[1] 陈骞[1] 杨婧[1] 赵梅凤 刘诗文 张示杰[1] YANG Zhao-hui;Deng Zi;ZHANG Hong-wei;CHU Zhi-qiang;ZHANG Long;CHEN Qian;YANG Jing;ZHAO Mei-feng;LIU Shi-wen;ZHANG Shi-jie(Department of Hepatobiliary Surgery,the First Affiliated Hospital of Shihezi University School of Medicine,Xinjiang Shihezi,832008;Department of Pathology,the First Affiliated Hospital of Shihezi University School of Medicine,Xinjiang Shihezi,832008)

机构地区：[1]石河子大学医学院第一附属医院肝胆外科,新疆石河子832008 [2]石河子大学医学院第一附属医院病理科,新疆石河子832008

出　　处：《农垦医学》2020年第3期220-223,共4页Journal of Nongken Medicine

基　　金：国家自然科学基金项目(817605569)。

摘　　要：目的:构建多房棘球蚴体外培养模型,观察体外泡状棘球蚴原头节与不同浓度肝癌细胞共培养后的生长情况。方法:提取原头节建立体外培养模型,分别与不同数量的大鼠肝癌细胞在DMEM培养基上共培养,连续观察三周其存活情况。结果:第1周Ⅰ组原头节在仅有DMEM培养基情况下明显的头节死亡,存活率仅为25.53%,Ⅱ组、Ⅲ组、Ⅳ组存活率分别为:68.62%、82.98%、69.23%,Ⅰ组较另三组比较均有统计学差异,三组之间存活率无统计学差异;第2周Ⅰ组原头节存活率为16.33%,Ⅱ组、Ⅲ组、Ⅳ组存活率分别为:55.32%、79.61%、41.18%,Ⅰ组较另外三组均有统计学差异,Ⅲ组与Ⅱ组和Ⅳ组均具有统计学差异,Ⅱ组和Ⅳ组之间存活率无统计学差异;第3周Ⅰ组原头节存活率仅6.12%,Ⅱ组、Ⅲ组、Ⅳ组存活率分别为:40.38%、75.47%、20.00%,Ⅲ组较Ⅱ在和Ⅳ组比较均具有统计学差异,Ⅱ组存活率与Ⅳ组存活率比较具有统计学差异。三周内四个组的原头节存活率随着时间的延长都逐渐下降,但Ⅰ组最为明显,其次Ⅳ组,随之Ⅱ组,Ⅲ组原头节生存率明显高于其他三组。结论:不同数量肝癌细胞对一定量原头节在体外存活的影响不同,2*10^6个大鼠肝癌细胞中原头节存活率最高。Objective:To study the growth of Echinococcus multilocularis protoscolex co-cultured with different concentrations of rat hepatoma cells,so as to improve the survival rate of protoscolex and provide experimental basis for the reduction of the interaction between host and parasite in vitro culture of Echinococcus multilocularis protoscolex.Methods:The protoscolex was extracted to establish the culture model in vitro,and the survival of hepatoma cells were observed on DMEM medium for three weeks.Results:In the first week,the survival rate of groupⅠwas only 25.53%when there was only DMEM medium.The survival rates of groupⅡ,groupⅢand groupⅣwere 68.62%,82.98%and 69.23%respectively.There were statistical differences between groupⅠand the other three groups.There was no statistical difference between the three groups.In the second week,the survival rate of groupⅠwas 16.33%,that of groupⅡ,groupⅢand groupⅣwas 55.32%,79.61%and 41.18%respectively.There was statistical difference between groupⅠand the other three groups.There was statistical difference between groupⅢand groupⅡand groupⅣ,and there was no statistical difference between groupⅡand groupⅣ.In the third week,groupⅠalmost died of the original scolex,and the survival rate was only 6.12%.The survival rates of groupⅡ,groupⅢand groupⅣwere 40.38%,75.47%and 20.00%respectively.The survival rates of groupⅢwere statistically different from those of groupⅡand GroupⅣ,and the survival rates of groupⅡand groupⅣwere statistically different.The survival rate of groupⅢwas significantly higher than that of the other three groups.Conclusion:The effect of different number of hepatocellular carcinoma cells on the survival of a certain amount of procephalin in vitro was different.The survival rate of protoscolex of 2*10~6 rat hepatoma cells is the highest.

关 键 词：原头节 肝癌细胞 共培养 存活率 

分 类 号：R383.33[医药卫生—医学寄生虫学]